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IL10 Mouse Elipair Kit (Reagents Provided for 10 x 96 well plates) (ab47600)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameIL10 Mouse Elipair Kit (Reagents Provided for 10 x 96 well plates)See all IL10 kits ...
  • Tests
    10 x 96 well plate
  • Assay typeELIPAIR
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The murine IL-10 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This Eli-pair set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Murine IL10

    Range: 31.25 ng/ml - 1000 ng/ml

    Sensitivity < 20 ng/ml

  • Tested applicationsELISA more details

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CSIFCytokine synthesis inhibitory factorIL-10
        IL10IL10_HUMANInterleukin 10Interleukin-10TGIF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab47600 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA: Use at an assay dependent dilution.


      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.

      • IL10 Mouse Elipair Kit (Reagents Provided for 10 x 96 well plates) images

      • - Murine IL10 Elipair Kit (ab47600)
        - Murine IL10 Elipair Kit (ab47600)
        Murine IL10 standard curve ranging from 31.25 to 1000 pg/mL

      References for IL10 Mouse Elipair Kit (Reagents Provided for 10 x 96 well plates) (ab47600)

      This product has been referenced in:
      • Syn WK  et al. Role for hedgehog pathway in regulating growth and function of invariant NKT cells. Eur J Immunol 39:1879-92 (2009). ELISA ; Mouse . Read more (PubMed: 19544307) »

      See 1 Publication for this product

      Publishing research using ab47600 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab47600 Submit a Question for ab47600

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

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      Hello. In the mouse IL-10 Elipair kit (ab47600) you provide with PVDF coated 96 well plates. Is there any other kind of plate I can use (e.g. tissue culture treated plates, or regular polysterene non coated plates)? Thankl you very much  

      Thank you for your inquiry. I can confirm that standard 96 wells polystyrene plates (or any other ELISPOT suitable plates) can be used with this kit. Optimal dilutions/concentrations should be determined by the end user since they might be different dep...

      Read More

      Phone call: Customer kindly asked about the plate they can used with this KIT ab47600.

      Thank you for contacting us. I can confirm that any Polystyrene ELISA plate can be used with ab47600. For best results I would recommend using the plate which have high binding capacity for proteins e.g. Nunc maxisorp cat #468667. I hope this info...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"