IL10 Mouse Elipair Kit (Reagents provided for 5 x 96 Well Plates) (ab47599)

Overview

  • Product nameIL10 Mouse Elipair Kit (Reagents provided for 5 x 96 Well Plates)See all IL10 kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The murine IL10 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This Eli-pair set is intended to measure murine IL10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Murine IL10

  • Tested applicationsELISAmore details
  • PlatformReagents

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL-10
    • IL10
    • IL10_HUMAN
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • IL10 Mouse Elipair Kit (Reagents provided for 5 x 96 Well Plates) images

    • Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL

    References for IL10 Mouse Elipair Kit (Reagents provided for 5 x 96 Well Plates) (ab47599)

    ab47599 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab47599.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"