Anti-IL10 antibody [2G101H7] (ab25073)

Overview

  • Product nameAnti-IL10 antibody [2G101H7]
    See all IL10 primary antibodies
  • Description
    Mouse monoclonal [2G101H7] to IL10
  • SpecificityRecognises recombinant and native IL10
  • Tested applicationsIHC-Fr, Dot Blot, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length protein (Rat)

  • Positive control
    • Stimulated rat peritoneal macrophages for flow cytometry.

Properties

Applications

Our Abpromise guarantee covers the use of ab25073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/250.
Dot Blot Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.
Flow Cyt Use 0.1µg for 106 cells.

Target

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSIF antibody
    • CSIF antibody
    • Cytokine Synthesis Inhibitory Factor antibody
    • Cytokine Synthesis Inhibitory Factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • Interleukin10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody [2G101H7] images

  • ab25073 staining IL10 in Rat hippocampal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 1.5% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in PBS) for 20 hours at 4°C. A DyLight®549-conjugated Horse anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-IL10 antibody [2G101H7] (ab25073)

ab25073 has not yet been referenced specifically in any publications.

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I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (hippocampus)
Specification hippocampus
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
Username

Dr. Nitsan Kozlovsky

Verified customer

Submitted Apr 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"