• Product nameAnti-IL10 antibody [2G101H7]
    See all IL10 primary antibodies
  • Description
    Mouse monoclonal [2G101H7] to IL10
  • SpecificityRecognises recombinant and native IL10
  • Tested applicationsIHC-Fr, Dot Blot, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length protein (Rat)

  • Positive control
    • Stimulated rat peritoneal macrophages for flow cytometry.
  • General notesAb81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



Our Abpromise guarantee covers the use of ab25073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/250.
Dot Blot Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.
Flow Cyt Use 0.1µg for 106 cells.


  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSIF antibody
    • CSIF antibody
    • Cytokine Synthesis Inhibitory Factor antibody
    • Cytokine Synthesis Inhibitory Factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • Interleukin10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody [2G101H7] images

  • ab25073 staining IL10 in Rat hippocampal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 1.5% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in PBS) for 20 hours at 4°C. A DyLight®549-conjugated Horse anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-IL10 antibody [2G101H7] (ab25073)

This product has been referenced in:
  • Yuan S  et al. Effect of bone marrow mesenchymal stem cell transplantation on acute hepatic failure in rats. Exp Ther Med 8:1150-1158 (2014). WB ; Rat . Read more (PubMed: 25187814) »

See 1 Publication for this product

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I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (hippocampus)
Specification hippocampus
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C

Dr. Nitsan Kozlovsky

Verified customer

Submitted Apr 12 2011