Anti-IL-10 antibody (ab9969)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: ELISA, WB, ICC/IF, Sandwich ELISA
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-IL-10 antibody
See all IL-10 primary antibodies -
Description
Rabbit polyclonal to IL-10 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Rat IL-10 aa 19-178.
(Peptide available asab9970) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970). |
ICC/IF
Use at an assay dependent concentration. |
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16153 Mouse
- Entrez Gene: 25325 Rat
- SwissProt: P18893 Mouse
- SwissProt: P29456 Rat
- Unigene: 874 Mouse
- Unigene: 9868 Rat
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (77)
ab9969 has been referenced in 77 publications.
- Peng L et al. Metformin alleviates prolonged isoflurane inhalation induced cognitive decline via reducing neuroinflammation in adult mice. Int Immunopharmacol 109:108903 (2022). PubMed: 35709590
- Zhou H et al. miR‑382 inhibits breast cancer progression and metastasis by affecting the M2 polarization of tumor‑associated macrophages by targeting PGC‑1α. Int J Oncol 61:N/A (2022). PubMed: 36069230
- Wang XK et al. TNAP-a potential cytokine in the cerebral inflammation in spastic cerebral palsy. Front Mol Neurosci 15:926791 (2022). PubMed: 36187348
- Setiawan E et al. Locally injected Mesenchymal Stem Cells optimize angiogenesis by regulating VEGF and CD31 expression in duodenal perforation. Ann Med Surg (Lond) 82:104529 (2022). PubMed: 36268307
- Sun Q et al. IRG1/itaconate increases IL-10 release to alleviate mechanical and thermal hypersensitivity in mice after nerve injury. Front Immunol 13:1012442 (2022). PubMed: 36311727