Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab76589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa).Can be blocked with Human IL11 peptide (ab90835).

Target

  • FunctionDirectly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P20809 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Adipogenesis inhibitory factor antibody
    • AGIF antibody
    • IL 11 antibody
    • IL-11 antibody
    • IL11 antibody
    • IL11_HUMAN antibody
    • Interleukin 11 antibody
    • Interleukin-11 antibody
    • Oprelvekin antibody
    see all

Anti-IL11 antibody images

  • Anti-IL11 antibody (ab76589) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • Anti-IL11 antibody (ab76589) at 1 µg/ml + Active human IL11 full length protein (ab73620) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Anti-IL11 antibody (ab76589)

This product has been referenced in:
  • Jiang Y  et al. Downregulation of SPARC expression inhibits the invasion of human trophoblast cells in vitro. PLoS One 8:e69079 (2013). WB . Read more (PubMed: 23935929) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"