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Overview

  • Product nameIL12 Human Elispot KitSee all IL12 kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeELISPOT
  • Species reactivity
    Reacts with: Human
  • Product overview

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principal:

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human IL12, specific to p70 heterodimer

  • Tested applicationsMeasuring secreted proteins from cells more details

Properties

  • RelevanceIL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. IL12B is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Cellular localizationSecreted
    • Alternative names
        CLMF1CLMF2IL 12 subunit p40
        IL 12AIL 12BIL12 subunit p35Interleukin 12AInterleukin 12BNFSK p35p40
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab62940 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISPOT ELISPOT

      References for IL12 Human Elispot Kit (ab62940)

      ab62940 has not yet been referenced specifically in any publications.

      Publishing research using ab62940 ? Please let us know so that we can cite the reference in this datasheet

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