IL12 Human Elispot Kit (ab62940)
- Product nameIL12 Human Elispot KitSee all IL12 kits ...
- Tests5 x 96 well plate
- Sample typeCell culture supernatant, Cell culture extracts
- Assay typeELISPOT
- Species reactivityReacts with: Human
- Product overview
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human IL12, specific to p70 heterodimer
- Tested applicationsMeasuring secreted proteins from cells more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 5 x 96 tests Bovine Serum albumin 1 x 1g Human IL12 Biotinylated detection antibody 1 x 0.55ml Human IL12 Pre-coated 96-well PDVF-bottomed plates 5 units Ready-to-use BCIP/NBT substrate buffer 1 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl
- CLMF1CLMF2IL 12 subunit p40
- IL 12AIL 12BIL12 subunit p35Interleukin 12AInterleukin 12BNFSK p35p40
Our Abpromise guarantee covers the use of ab62940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IL12 Human Elispot Kit (ab62940)
ab62940 has not yet been referenced specifically in any publications.