Anti-IL-12 antibody (ab9992)
Key features and details
- Goat polyclonal to IL-12
- Suitable for: ELISA, IHC-P, Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-IL-12 antibody
See all IL-12 primary antibodies -
Description
Goat polyclonal to IL-12 -
Host species
Goat -
Tested applications
Suitable for: ELISA, IHC-P, Sandwich ELISA, Neutralising, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human IL-12. p35: P29459 p40: P29460
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Positive control
- WB: Recombinant human IL-12 p40 protein (ab9993).
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
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IHC-P |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Notes |
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ELISA
Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
IHC-P
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161560 Human
- Omim: 161561 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
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Alternative names
- CLMF antibody
- CLMF p35 antibody
- CLMF p40 antibody
see all
Datasheets and documents
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Datasheet download
References (8)
ab9992 has been referenced in 8 publications.
- Zhang PP et al. COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy. Signal Transduct Target Ther 8:24 (2023). PubMed: 36609561
- Jia H et al. NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion. Neuropathology 42:181-189 (2022). PubMed: 35434787
- Feng C et al. Single-cell RNA sequencing reveals distinct immunology profiles in human keloid. Front Immunol 13:940645 (2022). PubMed: 35990663
- Leng H et al. Effects of aloe polysaccharide, a polysaccharide extracted from Aloe vera, on TNF-a-induced HaCaT cell proliferation and the underlying mechanism in psoriasis. Mol Med Rep 18:3537-3543 (2018). PubMed: 30066937
- Kang H et al. Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathway. Int J Oncol 50:545-554 (2017). PubMed: 28101568
- Cousin C et al. The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation. Eur J Immunol 45:1772-82 (2015). PubMed: 25778793
- Al-Sammak F et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . PubMed: 24069393
- Schlaepfer E & Speck RF Anti-HIV activity mediated by natural killer and CD8+ cells after toll-like receptor 7/8 triggering. PLoS ONE 3:e1999 (2008). Neutralising ; Human . PubMed: 18431484