Overview
Product nameIL12 p40 proteinSee all IL12 p40 proteins and peptides ...
Protein descriptionRecombinant full length Cow IL12 p40 (amino acids 23-327; 305aa) produced in yeast; Calculated MWt 34.6kDa (Swiss-Prot P46282).
Expression hostYeast
Properties
Purity> 95
% by SDS-PAGE
Purification notesab87464 is purified by Ion-exchange chromatography.
FormLyophilised:Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
Storage instructionsStore at -20°C. Stable for 12 months at -20°C
Storage bufferPreservative: None
Constituents: 10% Trehalose, 1.0X PBS
Concentration information loading...
Sequence notesMWELEKNVYV VELDWYPDAP GETVVLTCDT PEEDGITWTS DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEALSRSLL LLHKKEDGIW STDILKDQKE PKAKSFLKCE AKDYSGHFTC WWLTAISTDL KFSVKSSRGS SDPRGVTCGA ALLSAEKVSL EHREYNKYTV ECQEGSACPA AEESLLIEVV VEAVHKLKYE NYTSSFFIRD IIKPDPPKNL QLRPLKNSRQ VEVSWEYPDT WSTPHSYFSL TFCVQVQGKN KREKKLFMDQ TSAKVTCHKD ANVRVQARDR YYSSFWSEWA SVSCS
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab87464
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
CLMF p40CLMF2Cytotoxic lymphocyte maturation factor 40 kDa subunit
IL-12 subunit p40IL-12BIL12 subunit p40IL12BIL12B_HUMANInterleukin 12 p40Interleukin 12 subunit betaInterleukin 12BInterleukin-12 subunit betaNK cell stimulatory factor chain 2NKSFNKSF2p40
see all
FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Involvement in diseaseDefects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Post-translational
modificationsKnown to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Cellular localizationSecreted.
References for IL12 p40 protein (ab87464)
ab87464
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
