IL12 protein (Active) (ab89013)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab89013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: ab89013 is a disulfide-linked heterodimeric glycoprotein, consisting of an alpha and beta chain. ab89013 migrates as multiple bands between 30 and 45 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. ab89013 IL12 alpha chain migrates as bands between 30 and 35 kDa. This compares with the unmodified IL12 alpha chain that has a predicted molecular mass of 22.5 kDa. ab89013 beta chain migrates as bands between 35 and 45 kDa. This compares with the unmodified IL12 beta chain that has a predicted molecular mass of 34.7 kDa. ab89013 IL12 alpha chain consists of 15-35% carbohydrate by weight. ab89013 IL12 beta chain consists of 0-25% carbohydrate by weight.
pI: ab89013 separates into a number of isoforms with a pI between 3.5 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. ab89013 IL2 alpha chain separates into multiple isoforms with a pI between 3.5 and 9.5. This compares with the unmodified IL12 alpha chain that has a predicted pI of 6.1. ab89013 IL12 beta chain separates into multiple isoforms with a pI between 5.0 and 9.0. This compares with the unmodified IL12 beta chain that has a predicted pI of 5.4.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CLMFCLMF p35CLMF p40
- CLMF1CLMF2Cytotoxic lymphocyte maturation factor 1, p35Cytotoxic lymphocyte maturation factor 35 kDa subunitCytotoxic lymphocyte maturation factor 40 kDa subunitIL 12 subunit p35IL 12 subunit p40IL 2 subunit p40IL-12 subunit p35IL-12AIL-12BIL12, subunit p40IL12AIL12A_HUMANIL12BIL35 subunitInterleukin 12 alpha chainInterleukin 12 beta chainInterleukin 12 p35Interleukin 12 p40Interleukin 12 subunit betaInterleukin 12, p40Interleukin 12AInterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)Interleukin 12BInterleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)Interleukin-12 subunit alphaNatural killer cell stimulatory factor 1, 35 kD subunitnatural killer cell stimulatory factor 2Natural killer cell stimulatory factor, 40 kD subunitNF cell stimulatory factor chain 1NFSKNK cell stimulatory factor chain 1NK cell stimulatory factor chain 2NKSFNKSF1NKSF2p35p40
IL12 protein (Active) images
1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab89013
Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes.
A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg of protein was loaded. Spot trains indicate presence of multiple isoforms.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.
References for IL12 protein (Active) (ab89013)
ab89013 has not yet been referenced specifically in any publications.