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Overview

  • Product nameIL12 protein (Active)See all IL12 proteins and peptides ...
  • Protein descriptionRecombinant full length Human IL12 alpha (amino acids 23-219; 197aa) and IL12 beta (amino acids 23-328; 306aa) expressed in modified human 293 cells; Predicted MWt 22.5 kDa and 34.7 kDa respectively (Swiss-Prot P29459 and P29460).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence Alpha Chain: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQK ARQTLEFYPCTSEEIDHE DITKDKTSTVEACLPLELTK NESCLNSRETSFITNGSCLASRKTSFMMAL CLSSIYED LKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQAL NF NSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVT IDRVMSYLNAS Beta Chain: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPE EDGITWTLDQSSEVLGSG KTLTIQVKEFGDAGQYTCHK GGEVLSHSLLLLHKKEDGIWSTDILKDQKE PKNKTFLR CEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTC GA ATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPI EVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPL KNSRQVEVSWEYPDTWSTPHSYFSLT FCVQVQGKSKRE KKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEW ASVPCS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab89013 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab89013 is a disulfide-linked heterodimeric glycoprotein, consisting of an alpha and beta chain. ab89013 migrates as multiple bands between 30 and 45 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. ab89013 IL12 alpha chain migrates as bands between 30 and 35 kDa. This compares with the unmodified IL12 alpha chain that has a predicted molecular mass of 22.5 kDa. ab89013 beta chain migrates as bands between 35 and 45 kDa. This compares with the unmodified IL12 beta chain that has a predicted molecular mass of 34.7 kDa. ab89013 IL12 alpha chain consists of 15-35% carbohydrate by weight. ab89013 IL12 beta chain consists of 0-25% carbohydrate by weight.

    pI: ab89013 separates into a number of isoforms with a pI between 3.5 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. ab89013 IL2 alpha chain separates into multiple isoforms with a pI between 3.5 and 9.5. This compares with the unmodified IL12 alpha chain that has a predicted pI of 6.1. ab89013 IL12 beta chain separates into multiple isoforms with a pI between 5.0 and 9.0. This compares with the unmodified IL12 beta chain that has a predicted pI of 5.4.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CLMFCLMF p35CLMF p40
        CLMF1CLMF2Cytotoxic lymphocyte maturation factor 1, p35Cytotoxic lymphocyte maturation factor 35 kDa subunitCytotoxic lymphocyte maturation factor 40 kDa subunitIL 12 subunit p35IL 12 subunit p40IL 2 subunit p40IL-12 subunit p35IL-12AIL-12BIL12, subunit p40IL12AIL12A_HUMANIL12BIL35 subunitInterleukin 12 alpha chainInterleukin 12 beta chainInterleukin 12 p35Interleukin 12 p40Interleukin 12 subunit betaInterleukin 12, p40Interleukin 12AInterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)Interleukin 12BInterleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)Interleukin-12 subunit alphaNatural killer cell stimulatory factor 1, 35 kD subunitnatural killer cell stimulatory factor 2Natural killer cell stimulatory factor, 40 kD subunitNF cell stimulatory factor chain 1NFSKNK cell stimulatory factor chain 1NK cell stimulatory factor chain 2NKSFNKSF1NKSF2p35p40
      see all
  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P29459 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IL12 protein (Active) images

    • SDS-PAGE - IL12 protein (Active) (ab89013)
      SDS-PAGE - IL12 protein (Active) (ab89013)
      1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab89013
      Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

      Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes.
    • SDS-PAGE - IL12 protein (Active) (ab89013)
      SDS-PAGE - IL12 protein (Active) (ab89013)
      A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg of protein was loaded. Spot trains indicate presence of multiple isoforms.
    • Functional Studies - IL12 protein (Active) (ab89013)
      Functional Studies - IL12 protein (Active) (ab89013)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.

    References for IL12 protein (Active) (ab89013)

    ab89013 has not yet been referenced specifically in any publications.

    Publishing research using ab89013 ? Please let us know so that we can cite the reference in this datasheet

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