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Anti-IL13 antibody [32116.11] (ab10782)

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Overview

  • Product nameAnti-IL13 antibody [32116.11]See all IL13 primary antibodies ...
  • Description
    Mouse monoclonal [32116.11] to IL13
  • Tested applicationsWB, Neutralising, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human IL13 protein expressed in E. coli.

  • Positive controlab10782 has the ability to neutralize the bioactivity of human IL13 in the human erythroleukemia cell line, TF-1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThe antibody is purified from the IgG fraction of ascities fluid using Protein A chromotography.
  • Clonality Monoclonal
  • Clone number32116.11
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab10782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
Neutralising
ELISA
  • Application notesELISA: Use at a concentration of 4 µg/ml (100 ul/well) in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 µl/well), an ELISA for sample volumes of 100 µl with a range of 62.5-4000 ug/ml can be obtained.
    Neut: Use at a concentration of 1-3 µg/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1.
    WB: Use at a concentration of 1-2 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions.

    Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
    • Involvement in diseaseDefects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
    • Sequence similaritiesBelongs to the IL-4/IL-13 family.
    • Cellular localizationSecreted.

      • Target information above from: UniProt accession P35225 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Allergic rhinitis antibodyALRH antibodyBHR 1 antibody
          BHR1 antibodyBronchial hyperresponsiveness 1 (bronchial asthma) antibodyIL 13 antibodyIL-13 antibodyIL13 antibodyIL13_HUMAN antibodyInterleukin 13 antibodyInterleukin-13 antibodyInterleukin13 antibodyMGC116786 antibodyMGC116788 antibodyMGC116789 antibodyNC30 antibodyP600 antibody
        see all

      References for Anti-IL13 antibody [32116.11] (ab10782)

      ab10782 has not yet been referenced specifically in any publications.

      Publishing research using ab10782 ? Please let us know so that we can cite the reference in this datasheet

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      What is the antibody's affinity to its ligand?

      Thank you for your enquiry. Unfortunately the affinity constant for this antibody has not been determined. If you have any additional questions, please contact us again.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"