You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-IL13 antibodySee all IL13 primary antibodies ...
  • Description
    Rabbit polyclonal to IL13
  • Tested applicationsIndirect ELISA, Neutralising, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant Mouse IL13.

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab109815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA I-ELISA: Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL13.
Neutralising Neut: Use a concentration of 0.08 - 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL13 (10.0 ng/ml)
Sandwich ELISA sELISA: Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL13 (Biotin) (ab83162). Using ab83162 as the detection antibody and ab109815 as the capture antibody, the detection limit for recombinant Mouse IL13 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Mouse IL13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionCytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
  • Involvement in diseaseDefects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
  • Sequence similaritiesBelongs to the IL-4/IL-13 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P35225 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Allergic rhinitis antibodyALRH antibodyBHR1 antibody
        Bronchial hyperresponsiveness 1 (bronchial asthma) antibodyIL 13 antibodyIL-13 antibodyIl13 antibodyIL13_HUMAN antibodyInterleukin 13 antibodyInterleukin-13 antibodyMGC116786 antibodyMGC116788 antibodyMGC116789 antibodyNC30 antibodyP600 antibody
      see all

    References for Anti-IL13 antibody (ab109815)

    This product has been referenced in:
    • McSorley HJ  et al. Expansion of Foxp3+ regulatory T cells in mice infected with the filarial parasite Brugia malayi. J Immunol 181:6456-66 (2008). Read more (PubMed: 18941236) »

    See 1 Publication for this product

    Publishing research using ab109815 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab109815 Submit a Question for ab109815

    There are currently no Abreviews or Questions for ab109815.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"