Anti-IL-15RA antibody [JM7A4] (ab91270)
Key features and details
- Mouse monoclonal [JM7A4] to IL-15RA
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-IL-15RA antibody [JM7A4]
See all IL-15RA primary antibodies -
Description
Mouse monoclonal [JM7A4] to IL-15RA -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-15RA.
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Positive control
- LPS and IFN gamma stimulated PBMCs
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab91270 is affinity purified -
Clonality
Monoclonal -
Clone number
JM7A4 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use 0.5µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK. -
Tissue specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures. -
Sequence similarities
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated. -
Cellular localization
Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane. - Information by UniProt
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Database links
- Entrez Gene: 3601 Human
- Omim: 601070 Human
- SwissProt: Q13261 Human
- Unigene: 524117 Human
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Alternative names
- AA690181 antibody
- CD215 antibody
- I15RA_HUMAN antibody
see all
Images
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ab91270 staining IL-15RA in normal Human PBMCs by Flow cytometry.
Normal Human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-? for 24 hours.
Open/green histogram; stained with 0.25 µg of Purified Mouse IgG2b Isotype Control
Closed/blue histogram; stained with 0.25 µg of ab91270.
Detection utilised Biotin Anti-Mouse IgG and SAv-PE.
Cells in the large scatter population were used for analysis.
Datasheets and documents
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Datasheet download
References (3)
ab91270 has been referenced in 3 publications.
- Vidard L et al. CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation. J Immunol 203:676-685 (2019). PubMed: 31201235
- Vámosi G et al. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A 101:11082-7 (2004). PubMed: 15263076
- Dubois S et al. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity 17:537-47 (2002). PubMed: 12433361