Anti-IL1RA antibody (ab97301)
- Product nameAnti-IL1RA antibodySee all IL1RA primary antibodies ...
- DescriptionRabbit polyclonal to IL1RA
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 45 and 177 of IL1RA (NP_776214)
- Positive control293T, A431, HeLaS3, HepG2 and Raji cell lysate. Molt-4 whole cell lysate. HeLa cells.
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab97301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/3000. Predicted molecular weight: 20 kDa.|
|ICC/IF||ICC/IF: 1/100 - 1/200.|
- FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
- Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
- Involvement in diseaseGenetic variation in IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4) [MIM:612628]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Defects in IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]; also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis (bone inflammation in multiple places), periostitis (inflammation of the membrane surrounding the bones), and pustulosis (due to skin inflammation) from birth.
- Cellular localizationCytoplasm and Secreted.
- DIRA antibodyICIL 1RA antibodyICIL-1RA antibody
- ICIL1RA antibodyIL-1ra antibodyIL-1ra3 antibodyIL-1RN antibodyIL1 inhibitor antibodyIL1F3 antibodyIL1RA antibodyIL1RA_HUMAN antibodyIL1RN (IL1F3) antibodyIL1RN antibodyInterleukin 1 receptor antagonist antibodyInterleukin-1 receptor antagonist protein antibodyIntracellular IL 1 receptor antagonist type II antibodyIntracellular interleukin 1 receptor antagonist (icIL 1ra) antibodyIRAP antibodyMGC10430 antibodyMVCD4 antibodyType II interleukin 1 receptor antagonist antibody
Anti-IL1RA antibody images
Anti-IL1RA antibody (ab97301) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size : 20 kDa
Immunofluorescence analysis of methanol-fixed HeLa, using ab97301 at 1/200 dilution. Lower image costained with Hoechst 33342.
References for Anti-IL1RA antibody (ab97301)
ab97301 has not yet been referenced specifically in any publications.