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Peptide corresponding to amino acids 525 to 540of human IL-1RAcP, which is identical to those of mouseand rat origins (1).
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
Our Abpromise guarantee covers the use of ab8110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 66 kDa.Can be blocked with IL1RAP peptide (ab6104).|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration. PubMed: 17430890|
Immunohistochemical analysis of rat embryonic kidney tissue, staining IL1RAP with ab811 at 1/1000 dilution. Antigen retrieval was carried out by microwave oven in a citrate buffer.
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