Overview

  • Product nameAnti-IL1RAP antibody
    See all IL1RAP primary antibodies
  • Description
    Rabbit polyclonal to IL1RAP
  • SpecificityIt has no cross activity to other members in the IL-1 receptor family.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 525 to 540of human IL-1RAcP, which is identical to those of mouseand rat origins (1).

  • Positive control
    • HeLa whole cell lysate
  • General notes


    The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notes The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa.Can be blocked with IL1RAP peptide (ab6104).
IHC-P Use at an assay dependent dilution. PubMed: 17430890

Target

  • FunctionCoreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
  • Tissue specificityDetected in liver, skin, placenta, thymus and lung.
  • Sequence similaritiesBelongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3orf13 antibody
    • FLJ37788 antibody
    • IL 1 receptor accessory protein antibody
    • IL 1R 3 antibody
    • IL 1R3 antibody
    • IL 1RAcP antibody
    • IL 1RAP antibody
    • IL-1 receptor accessory protein antibody
    • IL-1R-3 antibody
    • IL-1R3 antibody
    • IL-1RAcP antibody
    • IL1 receptor accessory protein antibody
    • IL1AP_HUMAN antibody
    • IL1R3 antibody
    • IL1RAcP antibody
    • Il1rap antibody
    • Interleukin 1 Accessory Protein antibody
    • interleukin 1 receptor 3 antibody
    • Interleukin 1 receptor accessory protein antibody
    • interleukin 1 receptor accessory protein beta antibody
    • Interleukin-1 receptor 3 antibody
    • Interleukin-1 receptor accessory protein antibody
    see all

Anti-IL1RAP antibody images

  • Anti-IL1RAP antibody (ab8110) at 1/1000 dilution + HeLa whole cell lysate

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of rat embryonic kidney tissue, staining IL1RAP with ab811 at 1/1000 dilution. Antigen retrieval was carried out by microwave oven in a citrate buffer.

References for Anti-IL1RAP antibody (ab8110)

This product has been referenced in:
  • Planck SR  et al. Impact of IL-1 signalling on experimental uveitis and arthritis. Ann Rheum Dis 71:753-60 (2012). ICC/IF ; Mouse . Read more (PubMed: 22267332) »
  • Kim HS  et al. Identification of novel Wilms' tumor suppressor gene target genes implicated in kidney development. J Biol Chem 282:16278-87 (2007). IHC-P ; Rat . Read more (PubMed: 17430890) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"