Anti-IL1RAP antibody (ab8110)

Overview

• Product nameAnti-IL1RAP antibodySee all IL1RAP primary antibodies ...
• Description
  Rabbit polyclonal to IL1RAP
• SpecificityIt has no cross activity to other members in the IL-1 receptor family.
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Peptide corresponding to amino acids 525 to 540of human IL-1RAcP, which is identical to those of mouseand rat origins (1).

• Positive controlHeLa whole cell lysate
• General notes

  
  

  The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.02% sodium azide
• Concentration information loading...
• PurityImmunogen affinity purified
• Primary antibody notes The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI inresponse to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

Target

• FunctionCoreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
• Tissue specificityDetected in liver, skin, placenta, thymus and lung.
• Sequence similaritiesBelongs to the interleukin-1 receptor family.
  Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 TIR domain.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession Q9NPH3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3556 Human
  • Entrez Gene: 16180 Mouse
  • Entrez Gene: 25466 Rat
  • Omim: 602626 Human
  • SwissProt: Q9NPH3 Human
  • SwissProt: Q61730 Mouse
  • SwissProt: Q63621 Rat
  • Unigene: 478673 Human

  • Unigene: 253424 Mouse
  • Unigene: 10511 Rat

  see all

• Alternative names
  C3orf13 antibodyFLJ37788 antibodyIL 1 receptor accessory protein antibody

  IL 1R 3 antibodyIL 1R3 antibodyIL 1RAcP antibodyIL 1RAP antibodyIL-1 receptor accessory protein antibodyIL-1R-3 antibodyIL-1R3 antibodyIL-1RAcP antibodyIL1 receptor accessory protein antibodyIL1AP_HUMAN antibodyIL1R3 antibodyIL1RAcP antibodyIl1rap antibodyInterleukin 1 Accessory Protein antibodyinterleukin 1 receptor 3 antibodyInterleukin 1 receptor accessory protein antibodyinterleukin 1 receptor accessory protein beta antibodyInterleukin-1 receptor 3 antibodyInterleukin-1 receptor accessory protein antibody

  see all

Anti-IL1RAP antibody images

• 

  Western blot - IL1RAP antibody (ab8110)
  Anti-IL1RAP antibody (ab8110) at 1/1000 dilution + HeLa whole cell lysate
  
  Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1RAP antibody (ab8110)Image from Kim HS et al., J Biol Chem, 282, 16278-87. Fig 4.; doi: 10.1074/jbc.M700215200

  Immunohistochemical analysis of rat embryonic kidney tissue, staining IL1RAP with ab811 at 1/1000 dilution. Antigen retrieval was carried out by microwave oven in a citrate buffer.