Anti-IL-2 Receptor alpha antibody [7D4] (ab25370)
Key features and details
- Rat monoclonal [7D4] to IL-2 Receptor alpha
- Suitable for: IP, Flow Cyt
- Reacts with: Mouse
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [7D4]
See all IL-2 Receptor alpha primary antibodies -
Description
Rat monoclonal [7D4] to IL-2 Receptor alpha -
Host species
Rat -
Tested applications
Suitable for: IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse IL-2 Receptor alpha.
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Epitope
ab25370 reacts with an epitope between amino acids 125 and 212. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Purity
Purified IgM -
Clonality
Monoclonal -
Clone number
7D4 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16184 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab25370 has been referenced in 3 publications.
- Lorenzo F et al. Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping. J Immunol 147:2970-7 (1991). PubMed: 1717581
- Moreau JL et al. Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site. Eur J Immunol 17:929-35 (1987). PubMed: 2440696
- Ortega G et al. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J Immunol 133:1970-5 (1984). PubMed: 6206144