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IL2 Receptor beta protein (Active) (ab84068)

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Overview

  • Product nameIL2 Receptor beta protein (Active)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 27-237 of Human IL2 Receptor beta fused to the Fc region of human IgG1 (aa 93-330) expressed in modified human 293 cells (Swiss-Prot P14784).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityab84068 bound to protein A sepharose beads is able to pull down its ligand, IL2.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: AVNGTSQFTCFYNSRANISCVWSQDGALQ DTSCQVHAWPDRRRWNQ TCELLPVSQASWACNLILGAP DSQKLTTVDIVTLRVLCREGVRWRVMAI QDFKPFENLR LMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEART LSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVR VKPLQGEFTTW SPWSQPLAFRTKPAALGIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAK TKPRE EQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIE KTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKG FYPSDIAVEWESNG QPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALH NHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84068 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution. Migrates as a broad band between 65 and 85 kDa due to post-translation modifications, in particular glycosylation.

    Protein info

    • Alternative names
        CD122CD122 antigenHigh affinity IL 2 receptor beta subunit
        High affinity IL 2 receptor subunit betaHigh affinity IL-2 receptor subunit betaIL-2 receptor subunit betaIL-2R subunit betaIL-2RBIL15RBIL2 receptorIL2RBIL2RB_HUMANInterleukin 2 receptor betaInterleukin-2 receptor subunit betaP70 75P70-75P7075p75
      see all
  • FunctionReceptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P14784 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for IL2 Receptor beta protein (Active) (ab84068)

    ab84068 has not yet been referenced specifically in any publications.

    Publishing research using ab84068 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab84068 Submit a Question for ab84068

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    Inquiry: Hi, I'm looking to see if it is at all possible to get this material (ab84068) without added albumin. The carrier interferes with the assay I am trying to do. I don't need much, and I am local, and I am willing to have the protein in whatever for...

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    Thank you for contacting us.

    We cuurrently do not have this product in an albumin free state. The albumin is added by the originating lab as stabilizer and lyophilised prior to shipping. I am sorry for any inconvenience this may cause.
    ...

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    Does this protein retain function, in particular can the IL2 receptor beta still bind protein?

    This purifed IL-2beta receptor protein does bind IL-2 as shown by its ability to precipitate it whenab84068 is bound to protein A beads in an immunoprecipitation experiment. However it is not a fully functional receptor as ab84068 only contains the extrac...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"