IL2 Receptor gamma protein (Fc Chimera) (ab84065)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab84065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution. ab84065 migrates as a band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted MW of 55.1 kDa. ab84065 consists of 25-50% carbohydrate by weight.pI: ab84065 separates into a number of isoforms with a pI between 4.9 and 7.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 6.68.|
- CD132common cytokine receptor gamma chainCytokine receptor common subunit gamma
- Gamma Cgamma(c)gammaCIL-2 receptor subunit gammaIL-2R gamma chainIL-2R subunit gammaIL-2RGIL2RGIL2RG_HUMANIMD4interleukin 2 receptor, gammaInterleukin-2 receptor subunit gammaP64SCIDXSCIDX1
Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID) [MIM:312863]. XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID.
Contains 1 fibronectin type-III domain.
The box 1 motif is required for JAK interaction and/or activation.
IL2 Receptor gamma protein (Fc Chimera) images
1D SDS-PAGE of ab84065 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84065
Lane 2: ab84065 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84065 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
A sample of ab84065 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel.
40 µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms. Spots within the spot train were cut from the gel and identified as IL2 Receptor gamma (Fc Chimera) by protein mass fingerprinting.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
References for IL2 Receptor gamma protein (Fc Chimera) (ab84065)
ab84065 has not yet been referenced specifically in any publications.