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Overview

  • Product nameAnti-IL2 antibodySee all IL2 primary antibodies ...
  • Description
    Rabbit polyclonal to IL2
  • Tested applicationsWB, ELISA, Neutralising more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-2 (human Interleukin-2)

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent concentration. To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA ELISA: Use at an assay dependent concentration. To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-2.
Neutralising Neut: Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-2 (2.0 ng/ml), a concentration of 0.12 - 0.16 µg/ml of this antibody is required.

Target

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P60568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Aldesleukin antibodyIL 2 antibodyIL-2 antibody
        IL2 antibodyIL2_HUMAN antibodyInterleukin 2 antibodyInterleukin-2 antibodyInterleukin2 antibodyInvolved in regulation of T cell clonal expansion antibodyLymphokine antibodyOTTHUMP00000164090 antibodyPOIL2 antibodyT Cell Growth Factor antibodyT-cell growth factor antibodyTCGF antibody
      see all

    Anti-IL2 antibody images

    • Western blot - Anti-IL2 antibody (ab9618)
      Western blot - Anti-IL2 antibody (ab9618)
      All lanes : Anti-IL2 antibody (ab9618) at 1/1000 dilution

      Lane 1 : IL2 protein (Active) (ab73598) at 0.1 µg
      Lane 2 : IL2 protein (Active) (ab73598) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Anti-IL2 antibody (ab9618)

    ab9618 has not yet been referenced specifically in any publications.

    Publishing research using ab9618 ? Please let us know so that we can cite the reference in this datasheet

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    Customer is searching for an IL2 antibody that cross-reacts with human and can be used in a sandwich ELISA as both a capture and detection antibody.

    Thank you for your enquiry. Ab9618 has only been tested in a direct ELISA, as follows: To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity puri...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"