IL2 protein (Active) (ab83684)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab83684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
pI: ab83684 separates into a number of isoforms with a pI between 6.0 and 7.4 in
2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL2 that has a predicted pI of 7.05.
Molecular Mass: ab83684 migrates as a band between 15 and 18 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL2 that has a predicted molecular mass of 15.4 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- AldesleukinIL 2IL-2
- IL2IL2_HUMANInterleukin 2Interleukin-2Involved in regulation of T cell clonal expansionLymphokineOTTHUMP00000164090POIL2T Cell Growth FactorT-cell growth factorTCGF
IL2 protein (Active) images
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83684 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 ab83684
Lane 2 ab83684 treated with PNGase F to remove potential N-linked glycans
Lane 3 ab83684 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.
References for IL2 protein (Active) (ab83684)
ab83684 has not yet been referenced specifically in any publications.