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Product name
Anti-IL21 antibody
See all IL21 products (9) ...
Description
Rabbit polyclonal to IL21
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow
Immunogen
Synthetic peptide: TCPSCDSYEKKPPKE, corresponding to amino acids 121-135 of Human IL-21.
TCPSCDSYEKKPPKE
Positive control
WB: HL-60 or THP-1 whole cell lysate. ICC: K562 cells
General notes
Stable for one year.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> Cytokines >> Interleukins
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 2 µg/ml.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.Can be blocked with Il-21 Blocking Peptide (ab6025).
Target
Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
Sequence similarities
Belongs to the IL-15/IL-21 family.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9HBE4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IL 21 antibody
- IL-21 antibody
- Il21 antibody
see all
Database links
- Entrez Gene: 378475 Cow
- Entrez Gene: 59067 Human
- Entrez Gene: 60505 Mouse
- Omim: 605384 Human
- SwissProt: Q76LU5 Cow
- SwissProt: Q9HBE4 Human
- SwissProt: Q9ES17 Mouse
- Unigene: 302014 Human
see all
Anti-IL21 antibody images:
Western blot - IL21 antibody (ab5978)
Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml.
Western blot
Anti-IL21 antibody (ab5978) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 8 minutes
Immunocytochemistry/ Immunofluorescence - IL21 antibody (ab5978)
ab5978 at 2ug/ml staining of IL21 in K562 cells.
References for Anti-IL21 antibody (ab5978)
ab5978 has not yet been referenced specifically in any publications.
Publishing research using ab5978? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"