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Overview

  • Product nameAnti-IL33 antibodySee all IL33 primary antibodies ...
  • Description
    Rabbit polyclonal to IL33
  • Tested applicationsNeutralising, WB, Indirect ELISA, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human IL33.

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab110535 was purified by affinity chromatography employing immobilized hIL33 matrix.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab110535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Neutralising Neut: Use a concentration of 0.2 - 0.4 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL33 (1.0 ng/ml).
WB WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. The detection limit for recombinant hIL33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect ELISA I-ELISA: Use a concentration of 0.5 - 2 µg/ml. ab110535, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.
Sandwich ELISA sELISA: Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL33 (Biotin) (ab84249). ab110535, in conjunction with ab84249 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.

Target

  • FunctionCytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
  • Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
  • DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • Post-translational
    modifications    Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Cathepsin G and elastase can cleave IL33 and generate highly active forms in activated neutrophils (IL-33(95-270), IL-33(99-270) and IL-33(109-270)). Proteolysis is not strictly required for biological activity.
  • Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle > secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.

    • Target information above from: UniProt accession O95760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        C9orf26 antibodyCHROMOSOME 9 OPEN READING FRAME 26 antibodyDKFZp586H0523 antibody
        DVS27 antibodyDVS27 related protein antibodyIL 1F11 antibodyIL 33 antibodyIL-1F11 antibodyIL-33 antibodyIL1F11 antibodyIL33 antibodyIL33_HUMAN antibodyInterleukin 1 family member 11 antibodyInterleukin 33 antibodyINTERLEUKIN 33 NFHEV antibodyInterleukin 33 precursor antibodyInterleukin-1 family member 11 antibodyInterleukin-33 antibodyInterleukin33 antibodyNF HEV antibodyNF-HEV antibodyNFEHEV antibodyNFHEV antibodyNuclear factor for high endothelial venules antibodyNuclear factor from high endothelial venules antibodyOTTHUMP00000021041 antibodyRP11 575C20.2 antibody
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    References for Anti-IL33 antibody (ab110535)

    ab110535 has not yet been referenced specifically in any publications.

    Publishing research using ab110535 ? Please let us know so that we can cite the reference in this datasheet

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    I would like to know if I can use this for inhibition assays of human IL33. Should I block the activity of IL33 directly into cells in culture. What exactly this antibody also recognizes? Thanks

    Thank you for your enquiry and your interest in our products. I can confirm that this particular antibody (ab110535 - Anti-IL33 antibody) has been tested and characterized in neutralizing assay. As the datasheet indicates in order to yield one-half...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"