Overview

  • Product nameAnti-IL33 antibody
    See all IL33 primary antibodies
  • Description
    Rabbit polyclonal to IL33
  • Tested applicationsNeutralising, WB, Indirect ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human IL33.

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab110535 was purified by affinity chromatography employing immobilized hIL33 matrix.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab110535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use a concentration of 0.2 - 0.4 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL33 (1.0 ng/ml).
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. The detection limit for recombinant hIL33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. ab110535, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL33 (Biotin) (ab84249). ab110535, in conjunction with ab84249 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf26 antibody
    • CHROMOSOME 9 OPEN READING FRAME 26 antibody
    • DKFZp586H0523 antibody
    • DVS27 antibody
    • DVS27 related protein antibody
    • IL 1F11 antibody
    • IL 33 antibody
    • IL-1F11 antibody
    • IL-33 antibody
    • IL1F11 antibody
    • IL33 antibody
    • IL33_HUMAN antibody
    • Interleukin 1 family member 11 antibody
    • Interleukin 33 antibody
    • INTERLEUKIN 33 NFHEV antibody
    • Interleukin 33 precursor antibody
    • Interleukin-1 family member 11 antibody
    • Interleukin-33 (109-270) antibody
    • Interleukin33 antibody
    • NF HEV antibody
    • NF-HEV antibody
    • NFEHEV antibody
    • NFHEV antibody
    • Nuclear factor for high endothelial venules antibody
    • Nuclear factor from high endothelial venules antibody
    • OTTHUMP00000021041 antibody
    • RP11 575C20.2 antibody
    see all

References for Anti-IL33 antibody (ab110535)

ab110535 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and your interest in our products. I can confirm that this particular antibody (ab110535 - Anti-IL33 antibody) has been tested and characterized in neutralizing assay. As the datasheet indicates in order to yield one-h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"