Anti-IL33 antibody (ab110535)
- Product nameAnti-IL33 antibodySee all IL33 primary antibodies ...
- DescriptionRabbit polyclonal to IL33
- Tested applicationsNeutralising, WB, Indirect ELISA, Sandwich ELISA more details
- Species reactivityReacts with: Recombinant Fragment
Predicted to work with: Human
Highly pure (>98%) recombinant Human IL33.
- FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab110535 was purified by affinity chromatography employing immobilized hIL33 matrix.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab110535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Neut: Use a concentration of 0.2 - 0.4 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL33 (1.0 ng/ml).|
|WB||WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. The detection limit for recombinant hIL33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Indirect ELISA||I-ELISA: Use a concentration of 0.5 - 2 µg/ml. ab110535, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.|
|Sandwich ELISA||sELISA: Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL33 (Biotin) (ab84249). ab110535, in conjunction with ab84249 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.|
- FunctionCytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
- Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
- Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
- DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
modificationsProteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Cathepsin G and elastase can cleave IL33 and generate highly active forms in activated neutrophils (IL-33(95-270), IL-33(99-270) and IL-33(109-270)). Proteolysis is not strictly required for biological activity.
- Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle > secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.
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References for Anti-IL33 antibody (ab110535)
ab110535 has not yet been referenced specifically in any publications.