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Overview

  • Product nameIL33 protein (Active)See all IL33 proteins and peptides ...
  • Protein descriptionRecombinant full length Mouse IL33 (amino acids 109-266), untagged; Mwt: 18 kDa (UniProt ID = Q2YEJ4).
  • Uniprot accessionQ8BVZ5
  • Molecular weight18kDa
  • Protein length158 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivates ST2 dependent NF-kB pathway.
  • FormLyophilised:Reconstitute with 100 µl sterile water.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferConstituent: 99% PBS
  • Concentration information loading...
  • Additional notesPBS containing at least 0.1 % BSA should be used for further dilutions.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab109164 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        C9orf26CHROMOSOME 9 OPEN READING FRAME 26DKFZp586H0523
        DVS27DVS27 related proteinIL 1F11IL 33IL-1F11IL-33IL1F11IL33IL33_HUMANInterleukin 1 family member 11Interleukin 33INTERLEUKIN 33 NFHEVInterleukin 33 precursorInterleukin-1 family member 11Interleukin-33Interleukin33NF HEVNF-HEVNFEHEVNFHEVNuclear factor for high endothelial venulesNuclear factor from high endothelial venulesOTTHUMP00000021041RP11 575C20.2
      see all
  • FunctionCytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
  • Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
  • DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • Post-translational
    modifications    Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Cathepsin G and elastase can cleave IL33 and generate highly active forms in activated neutrophils (IL-33(95-270), IL-33(99-270) and IL-33(109-270)). Proteolysis is not strictly required for biological activity.
  • Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle > secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.

    • Target information above from: UniProt accession O95760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for IL33 protein (Active) (ab109164)

    ab109164 has not yet been referenced specifically in any publications.

    Publishing research using ab109164 ? Please let us know so that we can cite the reference in this datasheet

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