IL3RA protein (Fc Chimera) (ab88358)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab88358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: ab88358 migrates as a broad band between 85 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 60.6 kDa. ab88358 consists of 25-40% carbohydrate by weight.
pI: ab88358 separates into a number of isoforms with a pI between 6.2 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.43.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD123CD123 antigenhIL 3Ra
- hIL-3RahIL3RaIL 3 receptor alpha SP2 isoformIL 3R alphaIL-3 receptor subunit alphaIL-3R subunit alphaIL-3R-alphaIL-3RAIL3 Receptor alphaIL3RIL3RaIL3RA_HUMANIL3RAXIL3RAYIL3RXIL3RYInterleukin 3 receptor alpha chainInterleukin 3 receptor, alphaInterleukin 3 receptor, alpha (low affinity)Interleukin-3 Receptor alphaInterleukin-3 receptor subunit alphaInterleukin3 receptorInterleukin3 receptor, Y-chromosomal
The box 1 motif is required for JAK interaction and/or activation.
IL3RA protein (Fc Chimera) images
1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88358
Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
References for IL3RA protein (Fc Chimera) (ab88358)
ab88358 has not yet been referenced specifically in any publications.