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Overview

  • Product nameIL3RA protein (Fc Chimera)See all IL3RA proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 19-302 of Human IL3RA fused to the Fc region of human IgG1 (aa 93-330) expressed in modified human 293 cells, 533 amino acids, Predicted MWt 60.6 kDa (Swiss-Prot P26951).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTKEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADY SMPAVN NSYCQFGAISLCEVTNYTVRVANPPFSTWILF PENSGKPWAGAENL TCWIHDVDFLSCSWAVGPGAPADV QYDLYLNVANRRQQYECLHYKT DAQGTRIGCRFDDISR LSSGSQSSHILVRGRSAAFGIPCTDKFVVF SQIEILTP PNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQP VITEQVRDRTSFQLLNPGTYTVQIRARERVYEFLSAWSTP QRFECD QEEGANTRGGRVDGIQWIPKVDKKVEPKSCDK THTCPPCPAPELLG GPSVFLFPPKPKDTLMISRTPEVT CVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTI SKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab88358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab88358 migrates as a broad band between 85 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 60.6 kDa. ab88358 consists of 25-40% carbohydrate by weight.

    pI: ab88358 separates into a number of isoforms with a pI between 6.2 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.43.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD123CD123 antigenhIL 3Ra
        hIL-3RahIL3RaIL 3 receptor alpha SP2 isoformIL 3R alphaIL-3 receptor subunit alphaIL-3R subunit alphaIL-3R-alphaIL-3RAIL3 Receptor alphaIL3RIL3RaIL3RA_HUMANIL3RAXIL3RAYIL3RXIL3RYInterleukin 3 receptor alpha chainInterleukin 3 receptor, alphaInterleukin 3 receptor, alpha (low affinity)Interleukin-3 Receptor alphaInterleukin-3 receptor subunit alphaInterleukin3 receptorInterleukin3 receptor, Y-chromosomal
      see all
  • FunctionThis is a receptor for interleukin-3.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P26951 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IL3RA protein (Fc Chimera) images

    • SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
      SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
      1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab88358
      Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

      Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
    • SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
      SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
      A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
    • Functional Studies - IL3RA protein (Fc Chimera) (ab88358)
      Functional Studies - IL3RA protein (Fc Chimera) (ab88358)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MWt and pI of the protein.

    References for IL3RA protein (Fc Chimera) (ab88358)

    ab88358 has not yet been referenced specifically in any publications.

    Publishing research using ab88358 ? Please let us know so that we can cite the reference in this datasheet

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