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Overview

  • Product nameIL3RB protein (Fc Chimera)
  • Protein descriptionRecombinant full length extracellular domain of Human IL3RB (aa1-443) fused to the Fc region of Human IgG1 (aa 90-330) expressed in modified Human 293 cells. Swiss ID = P32927.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: WERSLAGAEETIPLQTLRCYNDYTSHITC RWADTQDAQRLVNVTLIRRVNEDL LEPVSCDLSDDMPW SACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGT RLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQS HWLSPGDLEFEV VYKRLQDSWEDAAILLSNTSQATLGP EHLMPSSTYVARVRTRLAPGSRLSGR PSKWSPEVCWDS QPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGL FYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPAT HGQYIVSVQPRRA EKHIKSSVNIQMAPPSLNVTKDGDS YSLRWETMKMRYEHIDHTFEIQYRKDTA TWKDSKTETL QNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEA R SWDTESVLPMWGSSNTKVDKKVEPKSCDKTHTCPPCP APELLGGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSH EDPEVKFNWYVDGVEVHNAKTKPREEQYN STYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREP QVYT LPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESN GQPENNYKTTPPVLDSDG SFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83919 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: Under reducing conditions ab83919 migrates as a broad band between 75 and 100 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL3RB-Fc Chimera that has a predicted monomeric molecular mass of 76 kDa.

    pI: ab83919 separates into a number of glycoforms with a pI between 5.5 and 7.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL3RB-Fc Chimera that has a predicted pI of 6.1.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD131CDw131Colony stimulating factor 2 receptor beta
        Colony stimulating factor 2 receptor beta low affinity (granulocyte macrophage)CSF2RBCytokine receptor common beta chainCytokine receptor common beta chain precursor (CDw131 antigen)Cytokine receptor common subunit betaGM CSF/IL 3/IL 5 receptor common beta chainGM-CSF/IL-3/IL-5 receptor common beta subunitIL3RBIL3RBIL3RB_HUMANIL5RBInterleukin 3 receptor/granulocyte macrophage colony stimulating factor 3 receptor beta (high affinity)stimulating factor 3 receptor, beta (high affinity)
      see all
  • FunctionHigh affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P32927 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IL3RB protein (Fc Chimera) images

    • Functional Studies - IL3RB protein (Fc Chimera) (ab83919)
      Functional Studies - IL3RB protein (Fc Chimera) (ab83919)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - IL3RB protein (Fc Chimera) (ab83919)
      SDS-PAGE - IL3RB protein (Fc Chimera) (ab83919)
      1D SDS-PAGE of ab83919 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83919
      Lane 2: ab83919 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab83919 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      Approximately 5 µg of protein was loaded per lane; Gel was stained using Deep Purple™.

    References for IL3RB protein (Fc Chimera) (ab83919)

    ab83919 has not yet been referenced specifically in any publications.

    Publishing research using ab83919 ? Please let us know so that we can cite the reference in this datasheet

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