IL4 Rat Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48424)

Overview

  • Product nameIL4 Rat Elipair Kit (Reagents for 10 x 96 Well Plates)See all IL4 kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    1000 ng/ml - 31.25 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    The rat IL-4 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour

    development step.

    This Eli-pair set is intended to measure rat IL-4 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure

    may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Rat IL4

    Range: 31.25 pg/ml - 1000 pg/ml

    Sensitivity < 15 pg/ml

  • Tested applicationsELISAmore details
  • PlatformReagents

Properties

  • RelevanceIL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • Cellular localizationSecreted
  • Alternative names
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • Interleukin 4
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • IL4 Rat Elipair Kit (Reagents for 10 x 96 Well Plates) images

    • Rat IL-4 Standard curve ranging from 31.25 to 1000 pg/mL

    References for IL4 Rat Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48424)

    ab48424 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"