IL4 protein (Active) (ab83686)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab83686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution. Migrates as a broad band between 15 and 20 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL4 that has a predicted molecular mass of 15.0 kDa.|
- B cell growth factor 1B cell IgG differentiation factorB Cell Stimulatory Factor 1
- B-cell stimulatory factor 1BCGF 1BCGF1BinetrakinBSF-1BSF1IGG1 induction factorIL 4IL-4IL4IL4_HUMANIl4e12Interleukin 4Interleukin 4, isoform 1Interleukin-4Lymphocyte stimulatory factor 1MGC79402Pitrakinra
IL4 protein (Active) images
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83686 before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.
Lane 1: ab83686
Lane 2: ab83686 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83686 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained.
Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for IL4 protein (Active) (ab83686)
ab83686 has not yet been referenced specifically in any publications.