IL4R protein (Active) (ab83756)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab83756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Under reducing conditions IL4R - Fc Chimera migrates as a broad band between 60 and 75 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL4R - Fc Chimera that has a predicted monomeric molecular mass of 51 kDa.
IL4R - Fc Chimera separates into a number of isoforms with a pI between 5.5 and 6.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL4R – Fc Chimera that has a predicted pI of 6.5.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD124IL 4R alphaIL-4 receptor subunit alpha
- IL-4-binding proteinIL-4R subunit alphaIL-4R-alphaIL-4RAIL4-BPIl4rIL4RAIL4RA_HUMANInterleukin 4 receptorInterleukin 4 receptor alpha chainsIL4Ralpha/protSoluble IL-4 receptor subunit alphaSoluble IL-4R-alphaSoluble interleukin-4 receptor subunit alpha
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Contains 1 fibronectin type-III domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsOn IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
IL4R protein (Active) images
Lane 1: MW markers; Lane 2: IL4R - Fc Chimera; Lane 3: IL4R - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4: IL4R - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.The triangle indicates theoretical pI and MW of the protein.
References for IL4R protein (Active) (ab83756)
ab83756 has not yet been referenced specifically in any publications.