IL5RA protein (Active) (ab85983)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab85983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Under reducing conditions IL5RA - Fc Chimera migrates as a broad band between 65 and 85 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL5RA - Fc Chimera that has a predicted monomeric molecular mass of 64 kDa.
IL5RA - Fc Chimera separates into a number of isoforms with a pI between 5.5 and 7.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL5RA – Fc Chimera that has a predicted pI of 6.2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD125CD125 antigenCDw125
- HSIL5R3IL-5 receptor subunit alphaIL-5R subunit alphaIL-5R-alphaIL-5RAIL5RIL5RAIL5RA_HUMANInterleukin 5 Receptor alphaInterleukin 5 Receptor alpha chainInterleukin 5 receptor alpha subunitInterleukin 5 receptor subunit alphaInterleukin 5 receptor type 3Interleukin-5 receptor subunit alphaMGC26560
The box 1 motif is required for JAK interaction and/or activation.
IL5RA protein (Active) images
Lane 1: MW markers; Lane 2: IL5RA - Fc Chimera; Lane 3: IL5RA - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4: IL5RA - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.The triangle indicates theoretical pI and MW of the protein.
References for IL5RA protein (Active) (ab85983)
ab85983 has not yet been referenced specifically in any publications.