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This Eli-pair set is intended to measure murine IL-6 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Principle of Method
The murine IL-6 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant Murine IL6
|Components||5 x 96 tests|
|Capture antibody Monoclonal anti-mIL6||1 x 0.1ml|
|Detection biotinylated antibody (Reconstitute with 0.55ml PBS 0.1% Azide w/v or another preservative)||1 x 0.55ml|
|Murine IL6 Eli-standard||5 vials|
|Ready-to-use TMB||2 vials|
Our Abpromise guarantee covers the use of ab47596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
ab47596 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"