You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

IL6 Rat ELISA Kit (with color giving dyes) (ab119548)

Submit an Abreview Q&A (1)

Overview

  • Product nameIL6 Rat ELISA Kit (with color giving dyes)See all IL6 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    12 pg/ml
  • Range
    31 pg/ml - 2000 pg/ml
  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab119548 IL6 Rat ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL6. ab119548 is for research use only and is not for diagnostic or therapeutic procedures.

    Interleukin-6 (IL6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms.

    IL6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections. IL6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells.

    IL6 is involved in

    - the induction of B-cell differentiation

    - the induction of acute phase proteins in liver cells

    - growth promotion of myeloma/plasmacytoma/hybridoma cells

    - induction of IL2 and IL2 receptor expression

    - proliferation and differentiation of T cells

    - inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages

    - enhancement of IL3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor

    - induction of mesangial cell growth

    - induction of neural differentiation of PC12 cells and

    - induction of keratinocyte growth

    The abnormal production of IL6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    N- and O-glycosylated.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        B cell differentiation factorB-cell stimulatory factor 2BSF-2
        BSF2CDFCTL differentiation factorHGFHSFHybridoma growth factorIFN-beta-2IFNB2IL-6IL6IL6_HUMANInterferon beta-2Interleukin 6Interleukin BSF2Interleukin-6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119548 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      IL6 Rat ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - IL6 Rat ELISA Kit (with color giving dyes) (ab119548)
        Sandwich ELISA - IL6 Rat ELISA Kit (with color giving dyes) (ab119548)
        Representative standard curve using ab119548.

      Protocols

      References for IL6 Rat ELISA Kit (with color giving dyes) (ab119548)

      ab119548 has not yet been referenced specifically in any publications.

      Publishing research using ab119548 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119548 Submit a Question for ab119548

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      What is the normal amount of rat IL4 in serum?
      What is the normal amount of rat IL6 in serum?
      What is the recommended dilution range?


      I have the following information for you regarding the kits we discussed:


      ab46073:
      We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living animal...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"