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Product name
Anti-IL6 antibody [MP5-20F3]
See all IL6 products (34) ...
Description
Rat monoclonal [MP5-20F3] to IL6
Specificity
Specifically neutralises mouse IL-6. It does not cross-react with LPS (the component of the bacterial cell wall which is considered responsible for the toxicity of gram-negative bacteria) and TNF alpha.
Tested applications
Inhibition Assay, Neutralisingmore details
Cross reactivity
Reacts with
Mouse
Does not react with
Rat, Human
General notes
For neutralisation of biological activity dilutions have to be made according to the amount of IL-6 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Phosphate buffered saline, 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
MP5-20F3
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Metabolic disorders
Metabolism >> Types of disease >> Cancer
Cancer >> Tumor immunology >> Cytokines >> Interleukins
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Neuroscience >> Neurology process >> Metabolism
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhibition Assay
Neutralising
Application notes
Inhib: Use at an assay dependent concentration.
Neut: Use at an assay dependent concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Neutralisation of mouse IL-6; For neutralisation of biological activity, dilutions have to be made according to the amount of IL-6 to be inactivated. Before using in biological assays, the product must be filter sterilized and depending on the concentration to be used, dialyzed against culture medium to remove the sodium azide added.
Inhibits IL-6-induced proliferation of the B9 assay.
Useful for immunochemical investigations of murine IL-6 in structural studies and for quantitative/qualitative immunochemical detection methods.
This antibody may be used as a coating antibody in EIA procedures.
Target
Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
Sequence similarities
Belongs to the IL-6 superfamily.
Post-translational
modifications
N- and O-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05231
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Database links
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
References for Anti-IL6 antibody [MP5-20F3] (ab7375)
ab7375 has not yet been referenced specifically in any publications.
Publishing research using ab7375? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"