Anti-IL6 antibody (ab66231)

Overview

• Product nameAnti-IL6 antibodySee all IL6 primary antibodies ...
• Description
  Mouse monoclonal to IL6
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human) with tag.

• Positive controlIL6 transfected 293T cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Angiogenic growth factors

  • Cancer
  • Tumor immunology
  • Cytokines
  • Interleukins

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
• Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
• Sequence similaritiesBelongs to the IL-6 superfamily.
• Post-translational
  modificationsN- and O-glycosylated.
• Cellular localizationSecreted.

Target information above from: UniProt accession P05231 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3569 Human
  • Omim: 147620 Human
  • SwissProt: P05231 Human
  • Unigene: 654458 Human

• Alternative names
  Interleukin BSF 2 antibodyB cell differentiation factor antibodyB cell stimulatory factor 2 antibody

  B-cell stimulatory factor 2 antibodyBSF 2 antibodyBSF-2 antibodyBSF2 antibodyCDF antibodyCTL differentiation factor antibodyCytotoxic T cell differentiation factor antibodyHepatocyte stimulating factor antibodyHGF antibodyHPGF antibodyHSF antibodyHybridoma growth factor antibodyHybridoma growth factor Interferon beta-2 antibodyHybridoma plasmacytoma growth factor antibodyIFN-beta-2 antibodyIFNB2 antibodyIL 6 antibodyIL-6 antibodyIL6 antibodyIL6 protein antibodyIL6_HUMAN antibodyInterferon beta 2 antibodyInterferon beta-2 antibodyInterleukin 6 antibodyInterleukin 6 (interferon beta 2) antibodyInterleukin-6 antibodyInterleukin6 antibody

  see all

Anti-IL6 antibody images

• 

  Western blot - IL6 antibody (ab66231)
  All lanes : Anti-IL6 antibody (ab66231) at 1 µg/ml
  
  Lane 1 : IL6 transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate.
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
  
  Predicted band size : 24 kDa
  Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.