Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab66231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).

Target

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Cytotoxic T cell differentiation factor antibody
    • Hepatocyte stimulating factor antibody
    • HGF antibody
    • HPGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • Hybridoma plasmacytoma growth factor antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6 protein antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    • Interleukin6 antibody
    see all

Anti-IL6 antibody images

  • All lanes : Anti-IL6 antibody (ab66231) at 1 µg/ml

    Lane 1 : IL6 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate.

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.

References for Anti-IL6 antibody (ab66231)

This product has been referenced in:
  • Feairheller DL  et al. Racial differences in oxidative stress and inflammation: in vitro and in vivo. Clin Transl Sci 4:32-7 (2011). WB ; Human . Read more (PubMed: 21348953) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab66231.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"