Overview
Product nameIL6 protein (Active)See all IL6 proteins and peptides ...
Protein descriptionRecombinant full length Human IL6 (amino acids 28-212) with a 16aa His tag produced in the endosperm tissue of barley grain (Hordeum vulgare); 201 amino acids with tag, Predicted MWt 23.1 kDa (UniProt P05231).
Expression hostBarley
Properties
Purity> 95
% by SDS-PAGE
Purification notesSterile filtered through a 0.2 µm filter.
Endotoxin level is less than 0.005ng per µg (0.05EU/µg) as measured by turbidimetric kinetic assay.
ab89582 carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL6, TNF-alpha and IL1beta induction.
Biological activityBioactivity is assayed by measuring the dose dependent effect of Human IL6 on proliferation of TF-1 cells. The ED50 is 0.70 ng/ml, corresponding to 1.4 x 106 U/mg specific activity.
FormLyophilised:Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage instructionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 500mM Sodium chloride, 50mM MES, pH 6.5
Concentration information loading...
Additional notesReconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab89582
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| Functional Studies |
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| SDS-PAGE |
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Application notesFuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 23-24 kDa in SDS-PAGE.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Interleukin BSF 2B cell differentiation factorB cell stimulatory factor 2
B-cell stimulatory factor 2BSF 2BSF-2BSF2CDFCTL differentiation factorHGFHSFHybridoma growth factorHybridoma growth factor Interferon beta-2IFN-beta-2IFNB2IL 6IL-6IL6IL6_HUMANInterferon beta 2Interferon beta-2Interleukin 6Interleukin 6 (interferon beta 2)Interleukin-6
see all
FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
Sequence similaritiesBelongs to the IL-6 superfamily.
Post-translational
modificationsN- and O-glycosylated.
Cellular localizationSecreted.
References for IL6 protein (Active) (ab89582)
ab89582
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
