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IL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates) (ab48796)

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Overview

  • Product nameIL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates)See all IL8 kits ...
  • Tests
    15 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 25 pg/ml
  • Range
    2000 pg/ml - 62.5 pg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    The IL8 Elipair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This Elipair set is intended to measure human IL8 in cell-culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Sensitivity: < 25 pg / ml

  • Tested applicationsELISA more details

Properties

  • RelevanceIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Cellular localizationSecreted
    • Alternative names
        CXCL8Interleukin 8MDNCF
        MONAPMonocyte derived neutrophil activating peptideMonocyte derived neutrophil chemotactic factorNAP1Neutrophil activating protein 1T cell chemotactic factor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab48796 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA: Use at an assay dependent dilution.


      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.

      • IL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates) images

      • Eli-pair - IL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates) (ab48796)
        Eli-pair - IL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates) (ab48796)
        IL-8 Standard curve ranging from 62.5 to 2000 pg/mL

      References for IL8 Human Elipair Kit (Reagents for 15 x 96 Well Plates) (ab48796)

      ab48796 has not yet been referenced specifically in any publications.

      Publishing research using ab48796 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab48796 Submit a Question for ab48796

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       Hello We are interested in ordering the following two kits: ab48796 and ab48772   I have following question, concerning theses kits: - you write that PVDF plates can be ordered additionally. We are always using the Maxisorb plates from Nunc to do ELISAs....

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      The references you mentioned are provided without buffers.Buffers are only included in our pre-coated ELISA Kit formats. The composition of the buffers are stated in the insert as follows: Normal 0 21 false false false MicrosoftInternetExplorer4 /* Style ...

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      We are interested in ordering the following two kits: ab48796 and ab48772   I have following question, concerning theses kits: - you write that PVDF plates can be ordered additionally. We are always using the Maxisorb plates from Nunc to do ELISAs. Are th...

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      Thank you for contacting us. Yes, the plates do not come with these kits. These products ab48796 and ab48772 only provide the reagents for 15 plates. Per information from the lab, any 96 wells polysterene plate (such as e.g. Nunc Maxisorp with catalog num...

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      What is the source/species/catalog number of the polyclonal detection antibody used in this kit?

      Thank you for your inquiry. The detection IL-8 antibody in ab48796 is a polyclonal raised in rabbit. It is specific for this kit, so we do not sell it separately with a different catalogue number. I hope this information helps. Please contact us...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"