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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab10768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use at an assay dependent concentration. The ND50 for this effect is approximately 0.08-0.4µg/ml in the presence of 0.02µg/ml of recombinant human IL8 using hCXCR-2 transfected BaF/3 cells.|
|ELISA||Use a concentration of 4 µg/ml.|
|Flow Cyt||Use a concentration of 25 µg/ml. For flow cytometry (intracellular staining), the cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin.
Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 11 kDa. The detection limit for in WB for recombinant human IL8 is approximately 0.5 ng/lane under non-reducing and reducing conditions in SDS PAGE.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"