• Product name
    Anti-IL8 antibody [6217]
    See all IL8 primary antibodies
  • Description
    Mouse monoclonal [6217] to IL8
  • Specificity
    By immunoblotting, the antibody shows less than 1% cross-reactivity with recombinant human GRO-beta under reducing conditions and less than 1% cross-reactivity with recombinant mouse JE under non-reducing conditions.
  • Tested applications
    Suitable for: Neutralising, ELISA, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control



Our Abpromise guarantee covers the use of ab10768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration. The ND50 for this effect is approximately 0.08-0.4µg/ml in the presence of 0.02µg/ml of recombinant human IL8 using hCXCR-2 transfected BaF/3 cells.
ELISA Use a concentration of 4 µg/ml.
Flow Cyt Use a concentration of 25 µg/ml. For flow cytometry (intracellular staining), the cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 11 kDa. The detection limit for in WB for recombinant human IL8 is approximately 0.5 ng/lane under non-reducing and reducing conditions in SDS PAGE.


  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all


This product has been referenced in:
  • Li Y  et al. Taxotere-induced elevated expression of IL8 in carcinoma-associated fibroblasts of breast invasive ductal cancer. Oncol Lett 13:1856-1860 (2017). WB ; Human . Read more (PubMed: 28454334) »
  • Wilson BJ  et al. ABCB5 maintains melanoma-initiating cells through a proinflammatory cytokine signaling circuit. Cancer Res 74:4196-207 (2014). Read more (PubMed: 24934811) »

See all 3 Publications for this product

Customer reviews and Q&As

Flow Cytometry
Mouse Cell (Mouse Peripheral Blood)
Yes - Fluidigm Fix and Perm Buffer
Gating Strategy
Positive cells for APC
Mouse Peripheral Blood
Cell harvesting/tissue preparation method: Retrobital bleeding to obtain peripheral blood from mice
Sample buffer: PBS + 10% Bovine Serum

Safa Mohamad

Verified customer

Submitted May 28 2016


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