Overview

  • Product nameAnti-IL8 antibody [807]
    See all IL8 primary antibodies
  • Description
    Mouse monoclonal [807] to IL8
  • SpecificityThis antibody recognizes both, recombinant and native human IL-8. This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
  • Tested applicationsELISA, Neutralising, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Purified recombinant human IL-8.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab18672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/ml of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect of MIP 1-beta and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

WB Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.

Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

IHC-Fr 1/2000.
IHC-P 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 18697197

Target

  • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 3 10C antibody
    • 310C antibody
    • 9E3 antibody
    • AMCF 1 antibody
    • AMCF1 antibody
    • AMCF1 antibody
    • b ENAP antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXCL 8 antibody
    • CXCL8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • Emoctakin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL 8 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • Inteleukin 8 antibody
    • Interleukin8 antibody
    • K 60 antibody
    • K60 antibody
    • K60 antibody
    • LECT antibody
    • LECT antibody
    • LUCT antibody
    • LUCT antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte derived neutrophil activating peptide antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP 1 antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB 8 antibody
    • SCYB8 antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG 1 antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody [807] images

  • ab18672 staining IL8 in inflammed human temporal artery by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an antigen retrieval step was performed using sodium citrate. Samples were then blocked with 5% serum for 1 hour at 25°C, then incubated with ab18672 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a rabbit anti-mouse polyclonal conjugated to biotin, 1/1000 dilution.

    See Abreview

  • ab18672 staining IL8 in porcine epithelial tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.25% Triton X-100, blocked with 10% serum for 2 hours at 25°C, then incubated with ab18672 at a 1/2000 dilution for 12 hours at 4°C. The secondary used was ab98640, used at a 1/1000 dilution.

    See Abreview

References for Anti-IL8 antibody [807] (ab18672)

This product has been referenced in:
  • Chen S & Barbieri JT Engineering botulinum neurotoxin to extend therapeutic intervention. Proc Natl Acad Sci U S A 106:9180-4 (2009). ELISA ; Human . Read more (PubMed: 19487672) »
  • Levina V  et al. Chemotherapeutic drugs and human tumor cells cytokine network. Int J Cancer 123:2031-40 (2008). ICC/IF, ELISA ; Human . Read more (PubMed: 18697197) »

See all 2 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Human IL-8 from R&D Systems)
Specification Human IL-8 from R&D Systems
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 07 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (tumor)
Specification tumor
Permeabilization Yes - PBS+0.3% Triton-X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 06 2013

Thank you for contacting us.
1. If you prefer to do the incubation at room temperature you should not exceed a 2 hour incubation. We do recommend an overnight incubation at 4 degrees, however, as this usually gives the strongest and most specific ...

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Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you

Credit note ID: 23605

The credit note ...

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Thank you for your reply.

I fully understand your concerns regarding the product code typing error on the datasheet, it is regrettable. I would like to reassure you that the image is from ab18672 IL8 antibody [807], and came from an Abreview...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer...

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Thank you for your enquiry.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

With regards to the text with the image, Ihave investigated thi...

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Thank you for contacting us.

Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

I am very sorry I couldn’t help you further. Please do not hesi...

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Thank you again for your call last week and for your patience while I have reviewed your images and these proteins.

For ab18672 (IL8), I think the diffuse bands are caused bya bittoo much proteinloaded in the lanes orfrom running the gel at t...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please send any images of the blots and we will see if there are some suggestions to improve the results. Could you also...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"