Overview

  • Product nameAnti-IL8 antibody [8M6]
    See all IL8 primary antibodies
  • Description
    Mouse monoclonal [8M6] to IL8
  • SpecificityRecognises ovine interleukin-8 and shows no cross-reactivity with ovine IL1 beta, IL6, MCP 1 or TNF alpha.
  • Tested applicationsFlow Cyt, WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Dog, Pig
  • Immunogen

    Recombinant full length protein (Sheep)

  • General notes


    Neutralises the bioactivity of ovine IL8 (removal of sodium azide is recommended prior to use in functional assays).

Properties

Applications

Our Abpromise guarantee covers the use of ab34100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. Use 10µl of the suggested working dilution to label 106 cells. Membrane permeabilisation is required.
WB Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.
ELISA Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21494875

Target

  • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 3 10C antibody
    • 310C antibody
    • 9E3 antibody
    • AMCF 1 antibody
    • AMCF1 antibody
    • AMCF1 antibody
    • b ENAP antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXCL 8 antibody
    • CXCL8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • Emoctakin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL 8 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • Inteleukin 8 antibody
    • Interleukin8 antibody
    • K 60 antibody
    • K60 antibody
    • K60 antibody
    • LECT antibody
    • LECT antibody
    • LUCT antibody
    • LUCT antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte derived neutrophil activating peptide antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP 1 antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB 8 antibody
    • SCYB8 antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG 1 antibody
    • TSG1 antibody
    see all

References for Anti-IL8 antibody [8M6] (ab34100)

This product has been referenced in:
  • Nakamura A  et al. Initial pulmonary respiration causes massive diaphragm damage and hyper-CKemia in Duchenne muscular dystrophy dog. Sci Rep 3:2183 (2013). WB . Read more (PubMed: 23851606) »
  • Cejková J  et al. Favorable effects of trehalose on the development of UVB-mediated antioxidant/pro-oxidant imbalance in the corneal epithelium, proinflammatory cytokine and matrix metalloproteinase induction, and heat shock protein 70 expression. Graefes Arch Clin Exp Ophthalmol 249:1185-94 (2011). IHC-Fr ; Rabbit . Read more (PubMed: 21494875) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA buffer pH 8.0. in pressure cooker
Sample Cow Tissue sections (Ovary)
Specification Ovary
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Natalia Salvetti

Verified customer

Submitted Oct 09 2014

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Expiration date: November 26, 2012

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