Anti-IL8 antibody [AS15] (Biotin) (ab16165)

Overview

  • Product nameAnti-IL8 antibody [AS15] (Biotin)See all IL8 primary antibodies ...
  • Description
    Mouse monoclonal [AS15] to IL8 (Biotin)
  • ConjugationBiotin
  • SpecificityDoes not recognise a panel of other human cytokines.
  • Tested applicationsELISAmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

Applications

Our Abpromise guarantee covers the use of ab16165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. This antibody has been used as a solution phase antibody at 2 µg/ml in a two site ELISA with unconjugated IL8 antibody. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml with the appropriate standard. Recovery of human IL8 in human serum is typically > 85% in the ELISA.

Target

  • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 3 10C antibody
    • 310C antibody
    • 9E3 antibody
    • AMCF 1 antibody
    • AMCF1 antibody
    • b ENAP antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXCL 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL 8 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • Interleukin8 antibody
    • K 60 antibody
    • K60 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte derived neutrophil activating peptide antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP 1 antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB 8 antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG 1 antibody
    • TSG1 antibody
    see all

References for Anti-IL8 antibody [AS15] (Biotin) (ab16165)

ab16165 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Human Recombinant protein (Human IL-8 from R&D Systems)
Specification Human IL-8 from R&D Systems
Type Sandwich (Detection)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Mar 04 2014

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