SpecificityThis antibody recognises both recombinant and native IL8. It does not cross react with Monocyte Chemotactic Activating Factor (MCAF)
or Regulated on Activation, Normal T-cell Expressed, and Secreted (RANTES).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Use at a concentration of 0.3 µg/well.
Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 11 kDa.
Use at an assay dependent dilution. Use at a concentration of 5 µg/ml with 10 ng/ml IL8 for 98.4% inhibition of chemotactic activity.
FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Post-translational modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.