Overview

  • Product nameAnti-IL8 antibodySee all IL8 primary antibodies ...
  • Description
    Goat polyclonal to IL8
  • SpecificityAnti-Human IL8 will neutralize the biological activity of both rhIL8 and natural human IL8. It will not neutralize the biological activity of rhGROalpha. By immunoblotting, the antibody shows <1% cross-reactivity with rhGROalpha, rhGRObeta, rhGROgamma, and MIP2. In addition, by ELISA, it shows no cross-reactivity with other cytokines tested.
  • Tested applicationsIHC-Fr, ICC/IF, Neutralising, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
  • General notesNeutralization: the antibody has the ability to neutralize the biological activity of natural and recombinant IL-8. To measure the ability of the antibody to neutralize the chemo-attractant activity of rhCXCL8 for BaF/3 cells transfected with hCXCR2 cells, rhIL-8 is preincubated with various dilutions of the antibody for 30 minutes at room temperature in a 96-well plate. Following this preincubation, 75 ul of the cytokine-antibody solution (containing rhIL-8 at a final concentration of 20 ng/mL and antibody at concentrations from 0.01-20 ug/ml) was transferred to the lower compartment of a 96 well chemotaxis chamber. The chamber was then assembled using a PVP-free polycarbonate filter (5 mm pore size) and 0.2 x 10E4 cells/well was added to the top chamber. After incubation for 3 hours at 37 °C in a 5% CO2 humidified incubator, the chamber was disassembled and the cells that migrated through to the lower chamber were transferred to a working plate and quantitated overnight. The fluorescence was then read in a fluorescent plate reader set at 544/590 nm. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhIL-8 that is present at a concentration just high enough to elicit a maximum response.

Properties

Applications

Our Abpromise guarantee covers the use of ab10769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 5 µg/ml.
ICC/IF Use a concentration of 5 - 15 µg/ml.
Neutralising Use a concentration of 0.01 - 20 µg/ml. Please see the General Notes section for detail.
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. Able to detect rhIL-8 at 5ng/lane under non-reducing and reducing condetions.

Target

  • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 3 10C antibody
    • 310C antibody
    • 9E3 antibody
    • AMCF 1 antibody
    • AMCF1 antibody
    • b ENAP antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXCL 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL 8 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • Interleukin8 antibody
    • K 60 antibody
    • K60 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte derived neutrophil activating peptide antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP 1 antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB 8 antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG 1 antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody images

  • ab10769 staining IL8 in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50 in culture medium) for 16 hours at 4°C. A Cy2®-conjugated Rabbit anti-goat polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-IL8 antibody (ab10769)

This product has been referenced in:
  • Stoll SJ  et al. The Transcription Factor HOXC9 Regulates Endothelial Cell Quiescence and Vascular Morphogenesis in Zebrafish via Inhibition of Interleukin 8. Circ Res 108:1367-77 (2011). WB ; Human . Read more (PubMed: 21493894) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

I was not able to obtain the ND50 concentration but our source for the antibody did narrow down the range of effective concentrations for neutralization. The neutralization dose of anti-IL8 antibody ab10769 is ty...

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Thank you for contacting us. This antibody is a total Goat IgG, so you could use a total IgG control such as ab37373.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informati...

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Thank you for your email. This inquiry makes more sense now. Currently we do not have anti IL8 products which had been tested in sELISA in combination with other antibodies. I am sorry I am unable to recommend any capture or detection antibody at th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEC)
Specification HUVEC
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Ms. Alice Hettler

Verified customer

Submitted Feb 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 100000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"