Overview

  • Product nameAnti-IL8 antibody
    See all IL8 primary antibodies
  • Description
    Rabbit polyclonal to IL8
  • SpecificityThe antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA.
  • Tested applicationsELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    recombinant human IL-8 produced in E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab6684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA (1:1,000 to 1:2,000)For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. Typically in ELISA this antiserum will detect IL-8 present in the supernatant or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS
  • Target

    • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications
      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • (Ala-IL-8)77 antibody
      • (Ser-IL-8)72 antibody
      • 3 10C antibody
      • 310C antibody
      • 9E3 antibody
      • AMCF 1 antibody
      • AMCF1 antibody
      • b ENAP antibody
      • Beta thromboglobulin like protein antibody
      • Beta thromboglobulin like protein antibody
      • C-X-C motif chemokine 8 antibody
      • CEF-4 antibody
      • chemokine, CXC motif, ligand 8 antibody
      • CXC chemokine ligand 8 antibody
      • CXCL 8 antibody
      • CXCL8 antibody
      • Emoctakin antibody
      • GCP 1 antibody
      • GCP-1 antibody
      • GCP/IL-8 protein I antibody
      • GCP/IL-8 protein II antibody
      • GCP/IL-8 protein III antibody
      • GCP/IL-8 protein IV antibody
      • GCP/IL-8 protein V antibody
      • GCP/IL-8 protein VI antibody
      • GCP1 antibody
      • Granulocyte chemotactic protein 1 antibody
      • IL 8 antibody
      • IL-8 antibody
      • IL-8(1-77) antibody
      • IL-8(9-77) antibody
      • IL8 antibody
      • IL8/NAP1 form I antibody
      • IL8/NAP1 form II antibody
      • IL8/NAP1 form III antibody
      • IL8/NAP1 form IV antibody
      • IL8/NAP1 form V antibody
      • IL8/NAP1 form VI antibody
      • IL8_HUMAN antibody
      • Inteleukin 8 antibody
      • Interleukin8 antibody
      • K 60 antibody
      • K60 antibody
      • LECT antibody
      • LUCT antibody
      • Lymphocyte derived neutrophil activating factor antibody
      • Lymphocyte-derived neutrophil-activating factor antibody
      • LYNAP antibody
      • MDNCF antibody
      • MDNCF-b antibody
      • MDNCF-c antibody
      • MONAP antibody
      • Monocyte derived neutrophil activating peptide antibody
      • Monocyte derived neutrophil activating protein antibody
      • Monocyte derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil-activating peptide antibody
      • NAF antibody
      • NAP 1 antibody
      • NAP-1 antibody
      • NAP1 antibody
      • Neutrophil activating factor antibody
      • Neutrophil activating peptide 1 antibody
      • Neutrophil activating protein 1 antibody
      • Neutrophil-activating factor antibody
      • Neutrophil-activating protein 1 antibody
      • Protein 3 10C antibody
      • Protein 3-10C antibody
      • SCYB 8 antibody
      • SCYB8 antibody
      • Small inducible cytokine subfamily B member 8 antibody
      • T cell chemotactic factor antibody
      • T-cell chemotactic factor antibody
      • TSG 1 antibody
      • TSG1 antibody
      see all

    References for Anti-IL8 antibody (ab6684)

    ab6684 has not yet been referenced specifically in any publications.

    Product Wall

    We currently have no data for this antibody concerning neutralisation.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"