Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 17208399
WB 1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.
IP 1/10.
ELISA Use at an assay dependent dilution.

Target

  • FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 3 10C antibody
    • 310C antibody
    • 9E3 antibody
    • AMCF 1 antibody
    • AMCF1 antibody
    • b ENAP antibody
    • Beta thromboglobulin like protein antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXC chemokine ligand 8 antibody
    • CXCL 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL 8 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • Interleukin8 antibody
    • K 60 antibody
    • K60 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte derived neutrophil activating factor antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte derived neutrophil activating peptide antibody
    • Monocyte derived neutrophil activating protein antibody
    • Monocyte derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP 1 antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating factor antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB 8 antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG 1 antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody images

  • All lanes : Anti-IL8 antibody (ab7747) at 1 µg/ml

    Lane 1 : Active human IL8 full length protein (ab73858) at 0.1 µg
    Lane 2 : Active human IL8 full length protein (ab73858) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 5000 µg/ml
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-IL8 antibody (ab7747)

This product has been referenced in:
  • Lin FY  et al. Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats. PLoS One 9:e102450 (2014). IHC-P ; Rat . Read more (PubMed: 25058444) »
  • Neri M  et al. Myocardial expression of TNF-alpha, IL-1beta, IL-6, IL-8, IL-10 and MCP-1 after a single MDMA dose administered in a rat model. Curr Pharm Biotechnol 11:413-20 (2010). IHC-P ; Rat . Read more (PubMed: 20420568) »

See all 7 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab7747 bisher noch nicht in Durchflusszytometrie getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein sp...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply.

In theory it is possible to perform IHC-P with a directly conjugated antibody. This will also mean that the amplification that is achieve by adding the secondary is lost.

So my advise would be to only do it...

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Thank you for your inquiry.

I am happy to confirm that the recommended protocol in IHC is the following:

Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

Antibody incubation: 120 min, 20C 1:500

(http...

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Thank you for your call today and for letting us know about the trouble with ab7747.

As we discussed, I'm sending a free of charge vial of ab18672 on the order *** which should arrive tomorrow.

Please keep me updated about the resul...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your protocol, I have looked at it in details and I would like to suggest trying the following changes to see if you start having a cytoplasmic staining rather than a nuclear staining. -check you are not overfixing (or underfixing) the...

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That is great to hear. Please contact us again with any more questions or comments.

The originator of ab7747 has suggested trying recombinant IL-8 to test your system or using activated macrophages. Since you don't have the original vial, do you have the Abcam order number or the purchase order number? Do you happen to know the lot nu...

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Thank you for your patience. I am working with the originator of ab7747 to help determine what may be going on with this particular antibody. I would like to confirm, with this batch of ab7747 you never saw an Il-8 band and the only band you saw was at...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"