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Overview

  • Product nameILF2 protein (Tagged-His Tag)See all ILF2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 25-390 (366 amino acids) of Human ILF2 with an N terminal His tag; Predicted MWt 41kDa (UniProt Q12905).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 108 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDL APNSAEQA SILSLVTKINNVIDNLIVAPGTFEVQIEEV RQVGSYKKGTMTTGHNVA DLVVILKILPTLEAVAALGN KVVESLRAQDPSEVLTMLTNETGFEISS SDATVKILIT TVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENAS QSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNN PTRQPLAL NVAYRRCLQILAAGLFLPGSVGITDPCESG NFRVHTVMTLEQQDMVCY TAQTLVRILSHGGFRKILGQ EGDASYLASEISTWDGVIVTPSEKAYEK PPEKKEGEEE EENTEEPPQGEEEESMETQE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91930 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        HGNC:6037ILF 2ilf2
        ILF2_HUMANInterleukin enhancer binding factor 2Interleukin enhancer binding factor 2, 45kDaInterleukin enhancer-binding factor 2MGC8391NF 45NF45Nuclear factor of activated T cells 45 kDaNuclear factor of activated T-cells 45 kDaPRO3063
      see all
  • FunctionAppears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • Sequence similaritiesContains 1 DZF domain.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    • Target information above from: UniProt accession Q12905 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ILF2 protein (Tagged-His Tag) images

    • SDS-PAGE - ILF2 protein (Tagged-His Tag) (ab91930)
      SDS-PAGE - ILF2 protein (Tagged-His Tag) (ab91930)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ILF2 protein (Tagged-His Tag) (ab91930)

    ab91930 has not yet been referenced specifically in any publications.

    Publishing research using ab91930 ? Please let us know so that we can cite the reference in this datasheet

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