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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-ILP2 antibody
Description
Rabbit polyclonal to ILP2
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: TGYEARLITFGT, corresponding to amino acids 2-13 of Human ILP2. (Peptide available as ab9665.)
TGYEARLITF GT
Positive control
Human HepG2 or MOLT4 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa. Can be blocked with ILP2 peptide (ab9665).
Human HepG2 or MOLT4 cell lysate can be used as a positive control.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Protects against apoptosis mediated by BAX.
Tissue specificity
Testis specific in normal tissues.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ96P09
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Baculoviral IAP repeat containing 8 antibody
- Baculoviral IAP repeat containing protein 8 antibody
- Baculoviral IAP repeat-containing protein 8 antibody
see all
Database links
- Entrez Gene: 112401 Human
- SwissProt: Q6IPY1 Human
- SwissProt: Q6PIA0 Human
- SwissProt: Q96P09 Human
- Unigene: 348263 Human
Anti-ILP2 antibody images:
Western blot - ILP2 antibody (ab9664)
Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates and mouse spleen (lane 3) tissue lysates with anti-ILP-2 at 1 µg/ml.
Immunocytochemistry - ILP2 antibody (ab9664)
ab9664 at 10µg/ml staining ILP2 in HepG2 cells by Immunocytochemistry.
References for Anti-ILP2 antibody (ab9664)
ab9664 has not yet been referenced specifically in any publications.
Publishing research using ab9664? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"