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Immunocytochemistry/ Immunofluorescence - INCENP antibody [58-217] (ab23956)
![Immunocytochemistry/ Immunofluorescence - INCENP antibody [58-217] (ab23956)](/ps/datasheet/images/ab23956-IF-Im1.jpg)
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Immunofluorescence - INCENP antibody [58-217] (ab23956)
![Immunofluorescence - INCENP antibody [58-217] (ab23956)](/ps/datasheet/images/ab23956_3.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - INCENP antibody [58-217] (ab23956)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - INCENP antibody [58-217] (ab23956)](/ps/datasheet/images/ab23956_4.jpg)
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Product Name
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INCENP antibody [58-217]
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See all INCENP antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [58-217] to INCENP
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Immunogen
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His-tagged human INCENP fragment aa231-298.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/200. Perform heat mediated antigen retrieval using citrate buffer pH 6.0 before commencing with IHC staining protocol.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Chromatin and Nuclear Signaling >> Chromosome Structure >> Centromeres
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Relevance
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Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex, highly basic, multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis. INCENP, in complex with the passenger proteins aurora B kinase and survivin, acts at multiple points during mitosis. Studies in knockout mice established INCENP as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis. Targeting of survivin to its centromeric and central spindle locations during mitosis is dependent on INCENP. Direct binding of INCENP to beta tubulin has been demonstrated. Interactions of INCENP with heterochromatin protein HP1Hsa alpha and histone H2A.Z has also been reported. INCENP levels are increased in several human cancer lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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58-217
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this INCENP antibody [58-217] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for INCENP antibody [58-217] images, references, products related to ab23956 and other tools.
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INCENP antibody [58-217] images:
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 Immunocytochemistry/ Immunofluorescence - INCENP antibody [58-217] (ab23956)
ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunofluorescence - INCENP antibody [58-217] (ab23956)
ab23956 staining INCENP in HeLa cells, by immunofluorescence. optimal antibody dilution: 1mg/ml optimal fixation protocol: PFA/Triton fixation: 10 min room temperature in 3,7 % PFA diluted in PHEM PEM buffer: 45 mM Hepes pH 6,9, 45 mM Pipes pH 6,9, 5mM MgCl2, 10 mM EGTA) containing 0 ,2% Triton X-100, followed by 3 washes in PBS. Also gives a good staining with PFA fixation: 10 min in 3,7 % PFA at room temperature, 5 min in PBS / 0,1%Triton X-100, then 3 washes in PBS. IF staining was performed following a standard protocol: blocking: 30 min; primary antibody: 1 hr; secondary antibody: 45’. All incubations were at 37 °C in PBS/ 0,1% Tween containing 3% BSA. This antibody was also tested using NIH-3T3 cells but it did not provide good results with these cells. Image kindly submitted by Serena Orlando, Giulia Guarguaglini and Patrizia Lavia, from the University 'La Sapienza' CNR, Italy
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - INCENP antibody [58-217] (ab23956)
ab23956 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-mouse antibody was used as the secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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Search PubMed (MEDLINE) for references to INCENP
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INCENP antibody [58-217] - more information
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Customer reviews (feedback) regarding INCENP antibody [58-217] |
Customer FAQs regarding INCENP antibody [58-217] |
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INCENP antibody [58-217] - related products:
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INCENP antibody [58-217] - other tools:
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Contact Abcam with a Technical Enquiry about INCENP antibody [58-217] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - INCENP antibody [58-217] (ab23956)](/index.html?datasheet=23956#ab23956-IF-Im1.jpg)
![Immunofluorescence - INCENP antibody [58-217] (ab23956)](/index.html?datasheet=23956#ab23956_3.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - INCENP antibody [58-217] (ab23956)](/index.html?datasheet=23956#ab23956_4.jpg)
