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ab12183 has been referenced in 8 publications.
Publishing research using ab12183? Please let us know so that we can cite the reference in this datasheet
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HeLa cells were fixed for 10mins in fresh 3.7% paraformaldehyde, permeabilised with PBS + 0.1% Triton X 100, and blocked with 1% goat serum in Abdil. The primary antibody (in Abdil) was incubated OVERNIGHT (1 hour didn't work) at room temperature at a dilution of 1/150. The cells were co-stained with mouse anti-tubulin (secondary is Anti-Mouse Alexa 488) and ACA (Human anti centromere marker, secondary is Anti-Human Cy5) and DAPI. The figure shows projected images of dividing cells (prophase, prometaphase, metaphase, anaphase, telophase). The top panels show ab12183, DNA and microtubule stains. The lower panels are the same cells, showing a subset of optical sections at higher magnification. ACA (geen) stains centromeres and ab12183 (red) stains the inner centromere in prophase, prometaphase, and metaphase. The ab12183 channel has been linerally scaled to reduce the background staining associated with this antibody.
This image was taken from an Abreview submitted on November 30, 2005 by William Moore. We do not have any further information relating to this image.
Demembranated sperm nuclei and CSF (cytostatic factor-arrested) low-speed egg extracts were prepared in XB and reacted at room temperature as described. Proteins were added to extract prior to sperm addition or else immediately after sperm addition into XB buffer (100 mM KCl, 1 mM MgCl2, 0.1 mM CaCl2, 10 mM K-HEPES pH 7.7, 50 mM sucrose) supplemented with energy mix (3.75 mM creatinine phosphate, 0.5 mM Na2-ATP, 0.5 mM MgCl2, 50 µM EGTA). To label damaged DNA, biotin-16-dUTP was added to metaphase reactions to a final concentration of 40 µM. 30 minutes after sperm addition, reactions were diluted 1/10 into XB supplemented with 1 mM MgCl2, 5 mM EGTA, 0.25% Triton X-100, and 10% formaldehyde and processed for Immunofluorescence. Antibody used ab12183 (red).
Image from BS Freedman et al, PLoS One 5: (2010), Fig 3.
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