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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
Overview
Product name
Anti-INCENP antibody
See all INCENP products (4) ...
Description
Rabbit polyclonal to INCENP
Tested applications
WB, ICC, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Predicted to work with
Chicken
Immunogen
Synthetic peptide: SKPRYHKRTSSAVWNSP (with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP.
SKPRYHKRTS SAVWNSP
Positive control
Nuclear/whole extract of human HeLa cells.
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000.Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC: 1/100
IP
IP: 1/250IP: 1/250
Application notes
Is unsuitable for or IF.
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
Sequence similarities
Belongs to the INCENP family.
Cellular localization
Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionQ9NQS7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- binds and activates aurora B and C in vivo and in vitro antibody
- Chromosomal passenger protein antibody
- FLJ31633 antibody
see all
Database links
- Entrez Gene: 396270 Chicken
- Entrez Gene: 3619 Human
- Entrez Gene: 16319 Mouse
- Omim: 604411 Human
- SwissProt: Q9NQS7 Human
- SwissProt: Q9WU62 Mouse
- Unigene: 1251 Chicken
- Unigene: 142179 Human
see all
Anti-INCENP antibody images:
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab12183)
HeLa cells were fixed for 10mins in fresh 3.7% paraformaldehyde, permeabilised with PBS + 0.1% Triton X 100, and blocked with 1% goat serum in Abdil. The primary antibody (in Abdil) was incubated OVERNIGHT (1 hour didn't work) at room temperature at a dilution of 1/150. The cells were co-stained with mouse anti-tubulin (secondary is Anti-Mouse Alexa 488) and ACA (Human anti centromere marker, secondary is Anti-Human Cy5) and DAPI. The figure shows projected images of dividing cells (prophase, prometaphase, metaphase, anaphase, telophase). The top panels show ab12183, DNA and microtubule stains. The lower panels are the same cells, showing a subset of optical sections at higher magnification. ACA (geen) stains centromeres and ab12183 (red) stains the inner centromere in prophase, prometaphase, and metaphase. The ab12183 channel has been linerally scaled to reduce the background staining associated with this antibody.
This image was taken from an Abreview submitted on November 30, 2005 by William Moore. We do not have any further information relating to this image.
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab12183)
Demembranated sperm nuclei and CSF (cytostatic factor-arrested) low-speed egg extracts were prepared in XB and reacted at room temperature as described. Proteins were added to extract prior to sperm addition or else immediately after sperm addition into XB buffer (100 mM KCl, 1 mM MgCl2, 0.1 mM CaCl2, 10 mM K-HEPES pH 7.7, 50 mM sucrose) supplemented with energy mix (3.75 mM creatinine phosphate, 0.5 mM Na2-ATP, 0.5 mM MgCl2, 50 µM EGTA). To label damaged DNA, biotin-16-dUTP was added to metaphase reactions to a final concentration of 40 µM. 30 minutes after sperm addition, reactions were diluted 1/10 into XB supplemented with 1 mM MgCl2, 5 mM EGTA, 0.25% Triton X-100, and 10% formaldehyde and processed for Immunofluorescence. Antibody used ab12183 (red).
Image from BS Freedman et al, PLoS One 5: (2010), Fig 3.
References for Anti-INCENP antibody (ab12183)
This product has been referenced in:
- Vázquez-Novelle MD & Petronczki M Relocation of the chromosomal passenger complex prevents mitotic checkpoint engagement at anaphase. Curr Biol 20:1402-7 (2010). ICC/IF; Human.Read more (PubMed: 20619651) »
- Freedman BSet al. Xenopus egg extracts increase dynamics of histone H1 on sperm chromatin. PLoS One 5: (2010). ICC/IF; Xenopus laevis.Read more (PubMed: 20927327) »
See all 8 publications for this product
Publishing research using ab12183? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"