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Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab12183)
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Product Name
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INCENP antibody
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See all INCENP antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to INCENP
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Immunogen
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Synthetic peptide: SKPRYHKRTSSAVWNSP (with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP.
BLAST 'SKPRYHKRTSSAVWNSP' with  or 
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Reacts with
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Hu, Ms, Rat, Xl
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Predicted to react
(species key)
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Chk
(Due to sequence homology)
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Tested applications
(see key)
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ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100.
IP: 1/250.
WB: Use at a minimum dilution of 1/2000 (recommended with whole extract of mouse NIH-3T3 and rat PC12 cells)- 1/5000 (recommended with a nuclear extract of human HeLa cells). Block with 5% milk for 2-24hrs. Incubate antibody at RT for 2hrs.
Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). Detection of the INCENP band by immunoblotting is specifically inhibited with the immunizing peptide.
There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells. However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Nuclear/whole extract of human HeLa cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Chromatin and Nuclear Signaling >> Chromosome Structure >> Centromeres
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Relevance
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Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex, highly basic, multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis. INCENP, in complex with the passenger proteins aurora B kinase and survivin, acts at multiple points during mitosis. Studies in knockout mice established INCENP as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis. Targeting of survivin to its centromeric and central spindle locations during mitosis is dependent on INCENP. Direct binding of INCENP to beta tubulin has been demonstrated. Interactions of INCENP with heterochromatin protein HP1Hsa alpha and histone H2A.Z has also been reported. INCENP levels are increased in several human cancer lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
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Form
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Liquid
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Concentration
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13.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this INCENP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for INCENP antibody images, references, products related to ab12183 and other tools.
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INCENP antibody images:
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 Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab12183)
HeLa cells were fixed for 10mins in fresh 3.7% paraformaldehyde, permeabilised with PBS + 0.1% Triton X 100, and blocked with 1% goat serum in Abdil.
The primary antibody (in Abdil) was incubated OVERNIGHT (1 hour didn't work) at room temperature at a dilution of 1/150. The cells were co-stained with mouse anti-tubulin (secondary is Anti-Mouse Alexa 488) and ACA (Human anti centromere marker, secondary is Anti-Human Cy5) and DAPI. The figure shows projected images of dividing cells (prophase, prometaphase, metaphase, anaphase, telophase). The top panels show ab12183, DNA and microtubule stains. The lower panels are the same cells, showing a subset of optical sections at higher magnification. ACA (geen) stains centromeres and ab12183 (red) stains the inner centromere in prophase, prometaphase, and metaphase. The ab12183 channel has been linerally scaled to reduce the background staining associated with this antibody. This image was taken from an Abreview submitted on November 30, 2005 by William Moore. We do not have any further information relating to this image.
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References for INCENP antibody (ab12183)
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This product has been used in: (two most recent references)
Andersen CB et al. Discovery of selective aminothiazole aurora kinase inhibitors. ACS Chem Biol 3:180-92 (2008). ICC/IF; Xenopus laevis. PubMed: 18307303
Yamamoto TM et al. Regulation of the Aurora B chromosome passenger protein complex during oocyte maturation in Xenopus laevis. Mol Cell Biol 28:4196-203 (2008). WB; Human. PubMed: 18378691
See all 4 publication references for this product.
If you publish research using ab12183 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to INCENP
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INCENP antibody - more information
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Customer reviews (feedback) regarding INCENP antibody |
Customer FAQs regarding INCENP antibody |
Protocols for INCENP antibody |
Price and availability of products related to INCENP antibody |
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INCENP antibody - related products:
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INCENP antibody - other tools:
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Contact Abcam with a Technical Enquiry about INCENP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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