Anti-INCENP antibody (ab36453)

Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

HeLa cells stained with ab36453 INCENP antibody diluted 1/1000 (green), anti-tubulin antibody (red) and DAPI to stain DNA (blue).

Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

ab36453 staining human (A431) epithelial cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% Triton X100 and blocked with 1% BSA for 30 minutes.  The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour.  An Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) was used as the secondary.  All antibody incubations and blocking was carried out at room temperature.

Legend: Green = tubulin, Pink = INCENP, Blue = DNA

This image is courtesy of an Abreview from Lux Fatimathas.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)

Ab36453 staining Human normal lung parenchyma. Staining is localised to nuclear and cytoplasmic compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.