|
|
|
|
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)
(enlarge)
|
|
|
|
|
Product Name
|
INCENP antibody
|
| |
See all INCENP antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to INCENP
|
|
Immunogen
|
Recombinant fusion protein (Human) consisting of the C-terminal 350 amino acids fused to a 6 x His tag
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ICC/IF, IHC-Fr, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: 1/1000. Fix cells in suspension by the addition of an equal volume of 4% paraformaldehyde prepared in phosphate-buffered saline (PBS, pH 7.4) for a final concentration of 2% paraformaldehyde. Fix cells at room temperature for 45 minutes and then centrifuge and washe twice with PBS. Resuspend cells in PBST (PBS containing 0.5% Triton-X100) with 3% bovine serum albumin (BSA) and incubate at room temperature for 15 to 30 minutes to provide blocking against nonspecific antibody reactivity (see Geddis et al).
IHC-Fr: 1/500.
IHC-P: 1/500.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Chromatin and Nuclear Signaling >> Chromosome Structure >> Centromeres
|
|
|
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Relevance
|
Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex, highly basic, multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis. INCENP, in complex with the passenger proteins aurora B kinase and survivin, acts at multiple points during mitosis. Studies in knockout mice established INCENP as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis. Targeting of survivin to its centromeric and central spindle locations during mitosis is dependent on INCENP. Direct binding of INCENP to beta tubulin has been demonstrated. Interactions of INCENP with heterochromatin protein HP1Hsa alpha and histone H2A.Z has also been reported. INCENP levels are increased in several human cancer lines.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Form
|
Liquid
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this INCENP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for INCENP antibody images, references, products related to ab36453 and other tools.
|
| |
INCENP antibody images:
|
 Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
HeLa cells stained with ab36453 INCENP antibody diluted 1/1000 (green), anti-tubulin antibody (red) and DAPI to stain DNA (blue).
|
| |
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
ab36453 staining human (A431) epithelial cells by ICC/IF. Cells were PFA fixed, permeabilized in 0.1% Triton X100 and blocked with 1% BSA for 30 minutes. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) was used as the secondary. All antibody incubations and blocking was carried out at room temperature. Legend: Green = tubulin, Pink = INCENP, Blue = DNA This image is courtesy of an Abreview from Lux Fatimathas.
See Abreview
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)
Ab36453 staining Human normal lung parenchyma. Staining is localised to nuclear and cytoplasmic compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
References for INCENP antibody (ab36453)
|
|
This product has been used in: (two most recent references)
Kim JB et al. Oct4-induced pluripotency in adult neural stem cells. Cell 136:411-9 (2009). ICC/IF; Human. PubMed: 19203577
Steigemann P et al. Aurora B-mediated abscission checkpoint protects against tetraploidization. Cell 136:473-84 (2009). PubMed: 19203582
See all 4 publication references for this product.
If you publish research using ab36453 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to INCENP
|
| |
INCENP antibody - more information
|
Customer reviews (feedback) regarding INCENP antibody |
Customer FAQs regarding INCENP antibody |
Protocols for INCENP antibody |
Price and availability of products related to INCENP antibody |
| |
INCENP antibody - related products:
|
|
|
| |
INCENP antibody - other tools:
|
Contact Abcam with a Technical Enquiry about INCENP antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
