Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
Overview
Product name
Anti-INCENP antibody
See all INCENP products (4) ...
Description
Rabbit polyclonal to INCENP
Tested applications
IHC-Fr, IHC-P, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fusion protein (Human) consisting of the C-terminal 350 amino acids fused to a 6 x His tag
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/500IHC-Fr: 1/500
IHC-P: 1/500
WB: 1/1000Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC/IF: 1/1000(PMID 15130946)
IP: Use at an assay dependent dilution.
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
Sequence similarities
Belongs to the INCENP family.
Cellular localization
Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionQ9NQS7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- binds and activates aurora B and C in vivo and in vitro antibody
- Chromosomal passenger protein antibody
- FLJ31633 antibody
see all
Database links
- Entrez Gene: 3619 Human
- Entrez Gene: 16319 Mouse
- Omim: 604411 Human
- SwissProt: Q9NQS7 Human
- SwissProt: Q9WU62 Mouse
- Unigene: 142179 Human
- Unigene: 29755 Mouse
Anti-INCENP antibody images:
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
HeLa cells stained with ab36453 INCENP antibody diluted 1/1000 (green), anti-tubulin antibody (red) and DAPI to stain DNA (blue).
Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)
ab36453 staining human (A431) epithelial cells by ICC/IF. Cells were PFA fixed, permeabilized in 0.1% Triton X100 and blocked with 1% BSA for 30 minutes. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) was used as the secondary. All antibody incubations and blocking was carried out at room temperature.
Legend: Green = tubulin, Pink = INCENP, Blue = DNA
This image is courtesy of an Abreview from Lux Fatimathas.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)
Ab36453 staining Human normal lung parenchyma. Staining is localised to nuclear and cytoplasmic compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-INCENP antibody (ab36453)
This product has been referenced in:
- Geddis AE & Kaushansky K Megakaryocytes express functional Aurora-B kinase in endomitosis. Blood 104:1017-24 (2004).Read more (PubMed: 15130946) »
- Wang Eet al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). WB, ICC/IF; Human.Read more (PubMed: 21844210) »
See all 8 publications for this product
Publishing research using ab36453? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"