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Anti-INCENP antibody (ab36453)

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Overview

Product name

Anti-INCENP antibody
See all INCENP products (4) ...

Description

Rabbit polyclonal to INCENP

Tested applications

IHC-Fr, IHC-P, WB, ICC/IF, IPmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant fusion protein (Human) consisting of the C-terminal 350 amino acids fused to a 6 x His tag

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

Sequence similarities

Belongs to the INCENP family.

Cellular localization

Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.

Target information above from: UniProt accessionQ9NQS7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • binds and activates aurora B and C in vivo and in vitro antibody
  • Chromosomal passenger protein antibody
  • FLJ31633 antibody
  • INCE_HUMAN antibody
  • INCENP antibody
  • Inner centromere protein antibody
  • Inner centromere protein antigens 135/155kDa antibody
  • Inner centromere protein antigens 135kD 155kD antibody
  • Inner centromere protein INCENP antibody
  • MGC111393 antibody
see all

Anti-INCENP antibody images:

  Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

HeLa cells stained with ab36453 INCENP antibody diluted 1/1000 (green), anti-tubulin antibody (red) and DAPI to stain DNA (blue).

  Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

Immunocytochemistry/ Immunofluorescence - INCENP antibody (ab36453)

ab36453 staining human (A431) epithelial cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% Triton X100 and blocked with 1% BSA for 30 minutes.  The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour.  An Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) was used as the secondary.  All antibody incubations and blocking was carried out at room temperature.

Legend: Green = tubulin, Pink = INCENP, Blue = DNA

This image is courtesy of an Abreview from Lux Fatimathas.

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-INCENP antibody(ab36453)

Ab36453 staining Human normal lung parenchyma. Staining is localised to nuclear and cytoplasmic compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-INCENP antibody (ab36453)

This product has been referenced in:

  • Geddis AE & Kaushansky K Megakaryocytes express functional Aurora-B kinase in endomitosis. Blood 104:1017-24 (2004).Read more (PubMed: 15130946) »
  • Wang Eet al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). WB, ICC/IF; Human.Read more (PubMed: 21844210) »

See all 8 publications for this product

Publishing research using ab36453? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"