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All lanes : Anti-ING3 antibody (ab3713) at 1/1000 dilution
Lane 1 : RKO whole cell lysate
Lane 2 : RKO cells transfected with ING3 - whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 800 conjugated Rabbit anti-Goat IgG
[H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
The identity of the non-specific bands at 33 and 20kDa has not been determined.
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